Rabbit Anti-ACAP2 Recombinant Antibody (clone R05-8H3) (CAT#: VS3-FY1683)
Clone R05-8H3 is a rabbit recombinant antibody that is capable of binding to human ACAP2 protein, a calculated weight of 88 kDa. It cross-reacts with hamster, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R05-8H3 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line U-2 OS shows localization to endosomes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/542_C10_2_selected.jpg

(Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34807/ihc_selected.jpg

(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/ihc_selected.jpg

(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_B_8_5.jpg

(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_7_3.jpg

(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_9_4.jpg

(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_9_5.jpg

(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_5_6.jpg

(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_9_8.jpg

(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000114331-ACAP2
Specifications
- Immunogen
- Synthetic peptide of human Centauri protein β2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster, Rat ACAP2
- Species Reactivity
- Human, Hamster, Rat
- Clone
- R05-8H3
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 88 kDa, while the observed molecular weight stands at 88 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- ArfGAP With Coiled-Coil, Ankyrin Repeat And PH Domains 2; Centaurin-Beta-2; CNT-B2; CENTB2; Arf-GAP With Coiled-Coil, ANK Repeat And PH Domain-Containing Protein 2; Arf GAP With Coiled Coil, ANK Repeat And PH Domains 2; Centaurin, Beta 2; KIAA0041
- Gene ID
- 23527
- UniProt ID
- Q15057
- Cellular Localization
- Endosome, Membrane
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- XP_006713620.1; XP_011510904.1; XP_011510905.1; XP_016861535.1; XP_016861536.1
- Function
- GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ACAP2"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0471CT | Mouse Anti-ACAP2 Recombinant Antibody (clone EML1406) | WB | Mouse IgG1 |
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