Rabbit Anti-ACAP2 Recombinant Antibody (clone R05-8H3) (CAT#: VS3-FY1683)
Clone R05-8H3 is a rabbit recombinant antibody that is capable of binding to human ACAP2 protein, a calculated weight of 88 kDa. It cross-reacts with hamster, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R05-8H3 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endosomes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/542_C10_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endosomes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/542_C10_2_selected.jpg
Figure 2 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34807/ihc_selected.jpg
(Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34807/ihc_selected.jpg
Figure 3 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/ihc_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_B_8_5.jpg
Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_7_3.jpg
Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_9_4.jpg
Figure 7 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_9_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_9_5.jpg
Figure 8 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_5_6.jpg
Figure 9 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34808/83547_A_9_8.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000114331-ACAP2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000114331-ACAP2
Specifications
- Immunogen
- Synthetic peptide of human Centauri protein β2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster, Rat ACAP2
- Species Reactivity
- Human, Hamster, Rat
- Clone
- R05-8H3
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 88 kDa, while the observed molecular weight stands at 88 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- ArfGAP With Coiled-Coil, Ankyrin Repeat And PH Domains 2; Centaurin-Beta-2; CNT-B2; CENTB2; Arf-GAP With Coiled-Coil, ANK Repeat And PH Domain-Containing Protein 2; Arf GAP With Coiled Coil, ANK Repeat And PH Domains 2; Centaurin, Beta 2; KIAA0041
- Gene ID
- 23527
- UniProt ID
- Q15057
- Cellular Localization
- Endosome, Membrane
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- XP_006713620.1; XP_011510904.1; XP_011510905.1; XP_016861535.1; XP_016861536.1
- Function
- GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ACAP2"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0471CT | Mouse Anti-ACAP2 Recombinant Antibody (clone EML1406) | WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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