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    Rabbit Anti-AHCY Recombinant Antibody (clone R35-5L-9) (CAT#: VS3-FY2133)

    The anti-AHCY antibody is a rabbit recombinant monoclonal antibody that binds to human AHCY protein, a calculated weight of 48 kDa. It cross-reacts with mouse, rat.
    The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R35-5L-9 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line CACO-2
    Figure 1 IF staining of human cell line CACO-2
    (Immunofluorescent staining of human cell line CACO-2 shows localization to cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/44675/if_selected.jpg
    Normal Tissue
    Figure 2 Lymph node
    Figure 2 Lymph node
    (Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/41225/87881_A_8_8.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/41225/87881_B_9_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/41225/87881_A_7_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/41225/87881_A_7_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/41225/87881_A_9_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/41225/87881_A_6_6.jpg
    Figure 8 Skin
    Figure 8 Skin
    (Fibroblasts Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/41225/87881_B_7_1.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101444-AHCY

    Specifications

    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse and Rat AHCY
    • Species Reactivity
    • Human, Mouse, Rat
    • Clone
    • R35-5L-9
    • Applications
    • Western Blot
    • Conjugate
    • Unconjugated
    • MW
    • The calculated weight of the target protein is approximately 48 kDa, while the observed molecular weight stands at 47kDa.
    • Related Disease
    • Hypermethioninemia With S-Adenosylhomocysteine Hydrolase Deficiency and S-Adenosylhomocysteine Hydrolase Deficiency

    Product Property

    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
    • Preservative
    • 0.02% Sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Western Blot: 1/500-1/1000

    Target

    • Alternative Names
    • Adenosylhomocysteinase; S-Adenosyl-L-Homocysteine Hydrolase; S-Adenosylhomocysteine Hydrolase; EC 3.3.1.1; AdoHcyase; SAHH
    • Sequence Similarities
    • Belongs to the adenosylhomocysteinase family.
    • Cellular Localization
    • Cytoplasm, Endoplasmic reticulum, Nucleus
    • Post Translation Modifications
    • Ubiquitination at Lys8, Lys20, Lys166, Lys188, Lys226, and Lys412 (NX_P23526 [NX_P23526-1])
      Modification sites at PhosphoSitePlus (P23526)
      Glycosylation from GlyGen (P23526) 2 sites, 1 O-linked glycan (2 sites)
    • Function
    • Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine.
      Binds copper ions (By similarity).

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    MOB-1056CT Mouse Anti-AHCY Recombinant Antibody (clone EML125) ELISA, WB Mouse IgM
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