Rabbit Anti-AHCY Recombinant Antibody (clone R35-5L-9)
CAT#: VS3-FY2133
The anti-AHCY antibody is a rabbit recombinant monoclonal antibody that binds to human AHCY protein, a calculated weight of 48 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R35-5L-9 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44675/if_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41225/87881_A_8_8.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41225/87881_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41225/87881_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41225/87881_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41225/87881_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41225/87881_A_6_6.jpg
Normal Tissue
Figure 8 Skin
Fibroblasts Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41225/87881_B_7_1.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101444-AHCY
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat AHCY
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R35-5L-9
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 48 kDa, while the observed molecular weight stands at 47kDa.
- Related Disease
- Hypermethioninemia With S-Adenosylhomocysteine Hydrolase Deficiency and S-Adenosylhomocysteine Hydrolase Deficiency
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Adenosylhomocysteinase; S-Adenosyl-L-Homocysteine Hydrolase; S-Adenosylhomocysteine Hydrolase; EC 3.3.1.1; AdoHcyase; SAHH
- Gene ID
- 191
- UniProt ID
- P23526
- Sequence Similarities
- Belongs to the adenosylhomocysteinase family.
- Cellular Localization
- Cytoplasm, Endoplasmic reticulum, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys8, Lys20, Lys166, Lys188, Lys226, and Lys412 (NX_P23526 [NX_P23526-1])
Modification sites at PhosphoSitePlus (P23526)
Glycosylation from GlyGen (P23526) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- XP_011526961.1; XP_016883198.1; XP_047295918.1; XP_054179113.1; XP_054179114.1
- Function
- Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine.
Binds copper ions (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "AHCY"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1056CT | Mouse Anti-AHCY Recombinant Antibody (clone EML125) | ELISA, WB | Mouse IgM |
| VS4-WK23 | Rabbit Anti-AHCY Recombinant Antibody (clone 13H8) | ELISA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0107 | Hi-Affi™ Rabbit Anti-AHCY Recombinant Antibody (clone DS107AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY62 | Anti-AHCY Immunohistochemistry Kit | IHC |
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