Anti-AP2A1 Immunohistochemistry Kit
CAT#: VS-0325-XY123
Our AP2A1 IHC Kit is designed to detect the AP2A1 protein with great precision. It has a comprehensive set of reagents, simplifying the experimental procedure. The user - friendly nature of the kit makes it suitable for studying AP2A1's functions in clathrin - mediated endocytosis and its role in receptor trafficking. This kit can be applied to paraffin sections and frozen sections.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4306/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4306/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4306/11687_B_9_5.jpg
Normal Tissue
Figure 4 Cerebellum
Cells in granular layer Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in molecular layer Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4306/11687_B_7_8.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4306/11687_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4306/11687_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4306/11687_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4306/11687_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196961-AP2A1
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse
- Target
- AP2A1
- Primary Antibody
- Rabbit Anti-AP2A1 Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "AP2A1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0191 | Hi-Affi™ Rabbit Anti-AP2A1 Recombinant Antibody (clone DS191AB) | ICC, IF, WB, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY428 | Anti-Mouse AP2A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY427 | Anti-Human AP2A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY429 | Anti-Bovine AP2A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY430 | Anti-Rat AP2A1 Immunohistochemistry Kit | IHC |
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