Rabbit Anti-AP2M1 (pT156) Recombinant Antibody (clone R03-8C7) (CAT#: VS3-WK1992)
This product is a rabbit recombinant antibody that is specific to phosphorylated AP2M1. The target of this antibody is phospho-AP2M1 (Thr156). The clone R03-8C7 can be used in WB analysis at a dilution of 1/500-1/1000. The molecular weight of AP2M1 (pThr156) observed in WB experiments using this antibody is 50 kDa.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69870/1336_C6_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69870/1336_C6_2_selected.jpg
Figure 2 Cerebral cortex
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_B_7_5.jpg
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_B_7_5.jpg
Figure 3 Cerebellum
(Cells in granular layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in molecular layer Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_B_9_8.jpg
(Cells in granular layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in molecular layer Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_B_9_8.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_A_9_3.jpg
Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_A_8_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_A_4_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_A_4_6.jpg
Figure 7 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_A_8_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_A_8_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161203-AP2M1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161203-AP2M1
Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Thr156 of human AP2M1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Thr156 of human AP2M1
- Species Reactivity
- Human
- Clone
- R03-8C7
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
- Alternative Names
- Adaptor Related Protein Complex 2 Mu 1 Subunit; HA2 50 KDA Subunit; Clathrin-Associated/Assembly/Adaptor Protein, Medium 1; Clathrin Assembly Protein Complex 2 Mu Medium Chain; Clathrin Assembly Protein Complex 2 Medium Chain; Adaptor-Related Protein Complex 2 Subunit Mu; Plasma Membrane Adaptor AP-2 50 KDa Protein; Plasma Membrane Adaptor AP-2 50kDA Protein; Clathrin Adaptor Complex AP2, Mu Subunit; Adaptor Protein Complex AP-2 Subunit Mu; Clathrin Coat-Associated Protein AP50; Clathrin Coat Assembly Protein AP50
- Gene ID
- 1173
- UniProt ID
- Q96CW1
- Long Name
- Adaptor Related Protein Complex 2 Mu 1 Subunit
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "AP2M1"
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Chicken IgY Antibody Products
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0783MZ | Chicken Anti-AP2M1 Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| BRD-0987MZ | Chicken Anti-AP2M1 Polyclonal IgY | WB | Chicken antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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