Rabbit Anti-AP2M1 (pT156) Recombinant Antibody (clone R03-8C7)
CAT#: VS3-WK1992
This product is a rabbit recombinant antibody that is specific to phosphorylated AP2M1. The target of this antibody is phospho-AP2M1 (Thr156). The clone R03-8C7 can be used in WB analysis at a dilution of 1/500-1/1000. The molecular weight of AP2M1 (pThr156) observed in WB experiments using this antibody is 50 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69870/1336_C6_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_B_7_5.jpg
Normal Tissue
Figure 3 Cerebellum
Cells in granular layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in molecular layer Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_B_9_8.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36849/79729_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161203-AP2M1
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Thr156 of human AP2M1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Thr156 of human AP2M1
- Species Reactivity
- Human
- Clone
- R03-8C7
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
- Alternative Names
- Adaptor Related Protein Complex 2 Mu 1 Subunit; HA2 50 KDA Subunit; Clathrin-Associated/Assembly/Adaptor Protein, Medium 1; Clathrin Assembly Protein Complex 2 Mu Medium Chain; Clathrin Assembly Protein Complex 2 Medium Chain; Adaptor-Related Protein Complex 2 Subunit Mu; Plasma Membrane Adaptor AP-2 50 KDa Protein; Plasma Membrane Adaptor AP-2 50kDA Protein; Clathrin Adaptor Complex AP2, Mu Subunit; Adaptor Protein Complex AP-2 Subunit Mu; Clathrin Coat-Associated Protein AP50; Clathrin Coat Assembly Protein AP50
- Gene ID
- 1173
- UniProt ID
- Q96CW1
- Long Name
- Adaptor Related Protein Complex 2 Mu 1 Subunit
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
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