This product is a recombinant antibody that recognizes APS. The monoclonal antibody CL1 reacts with the epitope of APS.
Figure 1 Four monoclonal aCL bind to human β2GP1 alone.
Antibodies were used at 1 mg/ml (filled and open bars) or 10 mg/ml (slashed bars; denoted Ab 10X), except for CL15 and CL24 (which were used at 2 mg/ml and 20 mg/ml, respectively).
Zhu, M. , Olee, T. , Le, D. T. , Roubey, R. A. S. , Hahn, B. H. , & Woods, J. V. L. , et al. (1999). Characterization of igg monoclonal anti-cardiolipin/anti-beta2gp1 antibodies from two patients with antiphospholipid syndrome reveals three species of antibodies. British Journal of Haematology, 105(1), 102-109.
Figure 2 Characterization of the serum cofactors for monoclonal aCL.
All antibodies were used at 1 mg/ml, except for CL15 and CL24 which were used at 2 mg/ml.
Zhu, M. , Olee, T. , Le, D. T. , Roubey, R. A. S. , Hahn, B. H. , & Woods, J. V. L. , et al. (1999). Characterization of igg monoclonal anti-cardiolipin/anti-beta2gp1 antibodies from two patients with antiphospholipid syndrome reveals three species of antibodies. British Journal of Haematology, 105(1), 102-109.
Figure 3 Effect of anti-β2-glycoprotein I (anti-β2GPI) IgG on the release of neutrophil extracellular traps (NETs) by neutrophils.
NET release from neutrophils stimulated with the indicated antibodies. Control neutrophils were treated with purified monoclonal antiphospholipid antibodies (aPL; 10 mg/ml) for 3 hours in the presence or absence of 10% autologous serum. IS4, aPL, CL1, and CL24 are known to bind b2GPI, while IS1 and IS2 do not.
Yalavarthi, S., Gould, T. J., Rao, A. N., Mazza, L. F., Morris, A. E., Núñez‐Álvarez, C., ... & Knight, J. S. (2015). Release of neutrophil extracellular traps by neutrophils stimulated with antiphospholipid antibodies: a newly identified mechanism of thrombosis in the antiphospholipid syndrome. Arthritis & rheumatology, 67(11), 2990-3003.
Figure 4 Stimulation of thrombin generation by purified antiphospholipid antibodies (aPL), as well as plasma from patients with antiphospholipid syndrome (APS), in a neutrophil- and DNA-dependent manner.
Representative thrombin (IIa) generation plot demonstrating enhanced generation when neutrophils are exposed to platelet-poor plasma (from a healthy control) supplemented with aPL CL1 (10 mg/ml).
Yalavarthi, S., Gould, T. J., Rao, A. N., Mazza, L. F., Morris, A. E., Núñez‐Álvarez, C., ... & Knight, J. S. (2015). Release of neutrophil extracellular traps by neutrophils stimulated with antiphospholipid antibodies: a newly identified mechanism of thrombosis in the antiphospholipid syndrome. Arthritis & rheumatology, 67(11), 2990-3003.
Figure 5 Blocking of antiphospholipid antibody (aPL)-mediated neutrophil extracellular trap (NET) release by inhibition of Toll-like receptor 4 (TLR-4) but not TLR-2.
Yalavarthi, S., Gould, T. J., Rao, A. N., Mazza, L. F., Morris, A. E., Núñez‐Álvarez, C., ... & Knight, J. S. (2015). Release of neutrophil extracellular traps by neutrophils stimulated with antiphospholipid antibodies: a newly identified mechanism of thrombosis in the antiphospholipid syndrome. Arthritis & rheumatology, 67(11), 2990-3003.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-74 | Recombinant Anti-APS Antibody | ELISA, WB, Neut, FuncS | IgG |
MHH-74 | Recombinant Human Anti-APS Antibody | ELISA, IP, FuncS | IgG |
HPAB-1958-FY | Human Anti-APS Recombinant Antibody (clone IS1) | ELISA, Activ | Human IgG |
HPAB-1959-FY | Human Anti-APS Recombinant Antibody (clone IS3) | ELISA | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-74-F(E) | Recombinant Anti-APS Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
MHH-74-F(E) | Recombinant Human Anti-APS Antibody Fab Fragment | RIA, IP, FuncS | Fab |
HPAB-1958-FY-F(E) | Human Anti-APS Recombinant Antibody (clone IS1); Fab Fragment | ELISA | Human Fab |
HPAB-1959-FY-F(E) | Human Anti-APS Recombinant Antibody (clone IS3); Fab Fragment | ELISA | Human Fab |
HPAB-1960-FY-F(E) | Human Anti-APS Recombinant Antibody (clone CL1); Fab Fragment | ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-74-S(P) | Recombinant Anti-APS Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
MHH-74-S(P) | Recombinant Human Anti-APS Antibody scFv Fragment | IP, IF, Biosensors, FuncS | scFv |
HPAB-1958-FY-S(P) | Human Anti-APS Recombinant Antibody (clone IS1); scFv Fragment | ELISA | Human scFv |
HPAB-1959-FY-S(P) | Human Anti-APS Recombinant Antibody (clone IS3); scFv Fragment | ELISA | Human scFv |
HPAB-1960-FY-S(P) | Human Anti-APS Recombinant Antibody (clone CL1); scFv Fragment | ELISA | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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