Rabbit Anti-ARHGAP26 Recombinant Antibody (clone R98-1X-5) (CAT#: VS3-FY2171)
This R98-1X-5 recombinant monoclonal antibody recognizes human ARHGAP26 protein, a calculated weight of 92 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R98-1X-5 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35106/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35106/if_selected.jpg
Figure 2 Cerebellum
(High expression in cerebellum)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35107/151371_B_7_8_val_selected.jpg
(High expression in cerebellum)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35107/151371_B_7_8_val_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35107/151371_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35107/151371_B_8_5.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35107/151371_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35107/151371_A_8_3.jpg
Figure 5 Gallbladder
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35107/151371_A_5_4.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35107/151371_A_5_4.jpg
Figure 6 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35107/151371_A_7_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35107/151371_A_7_5.jpg
Figure 7 RNA cell line category: Cell line enhanced (Karpas-707)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145819-ARHGAP26
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145819-ARHGAP26
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ARHGAP26
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R98-1X-5
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 92kDa, while the observed molecular weight stands at 100kDa.
- Related Disease
- Juvenile Myelomonocytic Leukemia and Myelodysplastic Syndrome
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Target
- Alternative Names
- Rho GTPase Activating Protein 26; Oligophrenin-1-Like Protein; OPHN1L; GRAF; GTPase Regulator Associated With Focal Adhesion Kinase Pp125(FAK); GTPase Regulator Associated With The Focal Adhesion Kinase Pp125; GTPase Regulator Associated With Focal Adhesion Kinase
- Gene ID
- 23092
- UniProt ID
- Q9UNA1
- Cellular Localization
- Cell junction, Cytoplasm, Cytoskeleton, Endosome, Membrane
- Post Translation Modifications
- Ubiquitination at Lys454 (NX_Q9UNA1 [NX_Q9UNA1-1])
Modification sites at PhosphoSitePlus (Q9UNA1)
- Protein Refseq
- XP_005268455.2; XP_005268456.2; XP_005268459.1; XP_006714837.2; XP_011535912.2
- Function
- GTPase-activating protein for RHOA and CDC42.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3247z-F(E) | Recombinant Anti-Human ARHGAP26 Antibody Fab Fragment | ELISA, Nert, FuncS | Fab |
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