Rabbit Anti-ARRB1 (pS412) Recombinant Antibody (clone R05-6F7)
CAT#: VS3-WK2021
This product is a rabbit recombinant antibody that is specific to phosphorylated ARRB1. The target of this antibody is phospho-ARRB1 (Ser412). The clone R05-6F7 can be used in WB analysis at a dilution of 1/500-1/1000, IP analysis at a dilution of 1/20, and IHC staining at a dilution of 1/50-1/100. The molecular weight of ARRB1 (pSer412) observed in WB experiments using this antibody is 50 kDa.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49318/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70249/164031_B_9_5_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3763/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70249/164031_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70249/164031_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70249/164031_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70249/164031_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70249/164031_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HEL, RH-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137486-ARRB1
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WB
Figure 1 Rabbit Anti-ARRB1 (pSer412) Recombinant Antibody (clone R05-6F7) in WB.
Western blot analysis of phospho-beta Arrestin 1 (Ser412) in A549, MCF-7, U251, HL-60 lysates using anti-ARRB1 (pSer412) antibody, Cat# VS3-WK2021.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Ser412 of human beta Arrestin 1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser412 of human ARRB1
- Species Reactivity
- Human
- Clone
- R05-6F7
- Applications
- WB, IHC-P, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IP: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "Arrb1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-682 | Mouse Anti-ARRB1 Recombinant Antibody (clone 30); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
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| PSBL-682 | Mouse Anti-ARRB1 Recombinant Antibody (clone 30); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0260 | Hi-Affi™ Rabbit Anti-ARRB1 Recombinant Antibody (clone DS260AB) | WB, FC, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0171-CN | Rabbit Anti-ARRB1 Recombinant Antibody (clone CBACN-068) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0519F | Mouse Anti-ARRB1 Recombinant Antibody (ZG-0519F) | WB | Mouse IgG |
| ZG-0520F | Mouse Anti-ARRB1 Recombinant Antibody (ZG-0520F) | WB | Mouse IgG |
| VS3-QX68 | Mouse Anti-ARRB1 Recombinant Antibody (clone 6A1-D7-C9) | WB | Mouse IgG2b |
| VS3-CJ988 | Rabbit Anti-ARRB1 Recombinant Antibody (VS3-CJ988) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY181 | Anti-ARRB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY536 | Anti-Human ARRB1 Immunohistochemistry Kit | IHC |
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