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    Rabbit Anti-ATE1 Recombinant Antibody (clone R09-6D4) (CAT#: VS3-FY1656)

    Clone R09-6D4 is a rabbit IgG recombinant monoclonal antibody that recognizes human ATE1 protein, a calculated weight of 59 kDa. It cross-reacts with rat.
    The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R09-6D4 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:50-1:100).

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    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/if_selected.jpg
    Figure 2 IF staining of human cell line U-251 MG
    Figure 2 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/57549/if_selected.jpg
    Normal Tissue
    Figure 3 IHC staining of human small intestine
    Figure 3 IHC staining of human small intestine
    (Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/ihc_selected.jpg
    Figure 4 Lymph node
    Figure 4 Lymph node
    (Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/80687_A_8_8.jpg
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/80687_B_7_5.jpg
    Figure 6 Thyroid gland
    Figure 6 Thyroid gland
    (Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/80687_B_3_5.jpg
    Figure 7 Colon
    Figure 7 Colon
    (Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/80687_A_8_3.jpg
    Figure 8 Liver
    Figure 8 Liver
    (Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/80687_A_9_4.jpg
    Figure 9 Kidney
    Figure 9 Kidney
    (Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/80687_A_9_5.jpg
    Figure 10 Testis
    Figure 10 Testis
    (Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/80687_A_6_6.jpg
    RNA Expression
    Figure 11 RNA cell line category: Low cell line specificity
    Figure 11 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107669-ATE1

    Specifications

    • Immunogen
    • Synthetic peptide of human ATE1
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human and Rat ATE1
    • Species Reactivity
    • Human, Rat
    • Clone
    • R09-6D4
    • Applications
    • Western Blot, Immunohistochemistry-Paraffin
    • MW
    • The calculated weight of the target protein is approximately 59 kDa, while the observed molecular weight stands at 59 kDa.
    • Related Disease
    • Johanson-Blizzard Syndrome and Chromosome 10Q26 Deletion Syndrome

    Product Property

    • Purification
    • Affinity Purified
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Western Blot: 1/500-1/1000
      Immunohistochemistry: 1/50-1/100

    Target

    • Alternative Names
    • Arginyltransferase 1; EC 2.3.2.8; Arginine-TRNA--Protein Transferase 1; R-Transferase 1
    • Sequence Similarities
    • Belongs to the R-transferase family.
    • Cellular Localization
    • Cytoplasm, Nucleus
    • Post Translation Modifications
    • Phosphoprotein
    • Function
    • Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues.

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "ATE1"

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    IgG Antibody Products
    CAT Product Name Application Type
    MOB-0697MZ Mouse Anti-ATE1 Recombinant Antibody (clone CBLS-130) ELISA Mouse IgG2b

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