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Rabbit Anti-ATE1 Recombinant Antibody (clone R09-6D4) (CAT#: VS3-FY1656)

Clone R09-6D4 is a rabbit IgG recombinant monoclonal antibody that recognizes human ATE1 protein, a calculated weight of 59 kDa. It cross-reacts with rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R09-6D4 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:50-1:100).


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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/if_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57549/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Figure 3 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/ihc_selected.jpg
Figure 4 Lymph node
Figure 4 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/80687_A_8_8.jpg
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/80687_B_7_5.jpg
Figure 6 Thyroid gland
Figure 6 Thyroid gland
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/80687_B_3_5.jpg
Figure 7 Colon
Figure 7 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/80687_A_8_3.jpg
Figure 8 Liver
Figure 8 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/80687_A_9_4.jpg
Figure 9 Kidney
Figure 9 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/80687_A_9_5.jpg
Figure 10 Testis
Figure 10 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38444/80687_A_6_6.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107669-ATE1

Specifications

  • Immunogen
  • Synthetic peptide of human ATE1
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human and Rat ATE1
  • Species Reactivity
  • Human, Rat
  • Clone
  • R09-6D4
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin
  • MW
  • The calculated weight of the target protein is approximately 59 kDa, while the observed molecular weight stands at 59 kDa.
  • Related Disease
  • Johanson-Blizzard Syndrome and Chromosome 10Q26 Deletion Syndrome

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100

Target

  • Alternative Names
  • Arginyltransferase 1; EC 2.3.2.8; Arginine-TRNA--Protein Transferase 1; R-Transferase 1
  • Sequence Similarities
  • Belongs to the R-transferase family.
  • Cellular Localization
  • Cytoplasm, Nucleus
  • Post Translation Modifications
  • Phosphoprotein
  • Function
  • Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues.

Recommended Products

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Recombinant Antibody

CAT Product Name Application Type
MOB-0697MZ Mouse Anti-ATE1 Recombinant Antibody (clone CBLS-130) ELISA Mouse IgG2b

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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