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Rabbit Anti-ATG4A Recombinant Antibody (clone R07-3J5) (CAT#: VS3-XY3270)

It is a recombinant rabbit antibody targeting the Autophagy related 4A cysteine peptidase (ATG4A), which catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy. It reacts with human, mouse and rat ATG4A. It has been determined using Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation.


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Specific Inquiry
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  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to microtubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64759/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Figure 2 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/ihc_selected.jpg
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_B_9_5.jpg
Figure 4 Colon
Figure 4 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_9_3.jpg
Figure 5 Liver
Figure 5 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_7_4.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_8_5.jpg
Figure 7 Testis
Figure 7 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_4_6.jpg
Figure 8 Skeletal muscle
Figure 8 Skeletal muscle
(Myocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_B_3_6.jpg
Figure 9 Lymph node
Figure 9 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HUVEC TERT2)
Figure 10 RNA cell line category: Cell line enhanced (HUVEC TERT2)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101844-ATG4A

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat ATG4A
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R07-3J5
  • Applications
  • WB, IHC-P, IP
  • Related Disease
  • Neurodegeneration With Brain Iron Accumulation

Product Property

  • Clonality
  • Monoclonal
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • WB: 1/500-1/1000
    IHC: 1/50-1/100
    IP: 1/20

Target

  • Alternative Names
  • APG4A; AUTL2; HsAPG4A
  • Long Name
  • Autophagy related 4A cysteine peptidase
  • Cellular Localization
  • Cytoplasm
  • Post Translation Modifications
  • Modification sites at PhosphoSitePlus (Q8WYN0)
    Modification sites at neXtProt (NX_Q8WYN0)
  • Function
  • Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.
    Involved in phagophore growth during mitophagy independently of its protease activity and of ATG8 proteins: acts by regulating ATG9A trafficking to mitochondria and promoting phagophore-endoplasmic reticulum contacts during the lipid transfer phase of mitophagy.

Recommended Products

Isotype Control

Recommended Dilution Buffer

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "ATG4A"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0280 Hi-Affi™ Rabbit Anti-ATG4A Recombinant Antibody (clone DS280AB) IHC-P, IP, WB Rabbit IgG

Recombinant Antibody

CAT Product Name Application Type
VS3-QX73 Mouse Anti-ATG4A Recombinant Antibody (clone 1458CT808.66.25.69) WB, ICC, IHC, FC Mouse IgG2b
VS3-WK809 Rabbit Anti-ATG4A Recombinant Antibody (clone VS3-WK809) IHC, IF, ELISA Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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