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    Rabbit Anti-ATG4A Recombinant Antibody (clone R07-3J5) (CAT#: VS3-XY3270)

    It is a recombinant rabbit antibody targeting the Autophagy related 4A cysteine peptidase (ATG4A), which catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy. It reacts with human, mouse and rat ATG4A. It has been determined using Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation.

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    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line PC-3
    Figure 1 IF staining of human cell line PC-3
    (Immunofluorescent staining of human cell line PC-3 shows localization to microtubules.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64759/if_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human pancreas
    Figure 2 IHC staining of human pancreas
    (Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/ihc_selected.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_B_9_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_9_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_7_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_8_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_4_6.jpg
    Figure 8 Skeletal muscle
    Figure 8 Skeletal muscle
    (Myocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_B_3_6.jpg
    Figure 9 Lymph node
    Figure 9 Lymph node
    (Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_9_8.jpg
    RNA Expression
    Figure 10 RNA cell line category: Cell line enhanced (HUVEC TERT2)
    Figure 10 RNA cell line category: Cell line enhanced (HUVEC TERT2)
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101844-ATG4A

    Specifications

    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse and Rat ATG4A
    • Species Reactivity
    • Human, Mouse, Rat
    • Clone
    • R07-3J5
    • Applications
    • WB, IHC-P, IP
    • Related Disease
    • Neurodegeneration With Brain Iron Accumulation

    Product Property

    • Clonality
    • Monoclonal
    • Aggregation
    • < 5%
    • Format
    • Liquid
    • Buffer
    • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
    • Preservative
    • 0.02% sodium azide
    • Storage
    • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • WB: 1/500-1/1000
      IHC: 1/50-1/100
      IP: 1/20

    Target

    • Alternative Names
    • APG4A; AUTL2; HsAPG4A
    • Long Name
    • Autophagy related 4A cysteine peptidase
    • Cellular Localization
    • Cytoplasm
    • Post Translation Modifications
    • Modification sites at PhosphoSitePlus (Q8WYN0)
      Modification sites at neXtProt (NX_Q8WYN0)
    • Function
    • Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.
      Involved in phagophore growth during mitophagy independently of its protease activity and of ATG8 proteins: acts by regulating ATG9A trafficking to mitochondria and promoting phagophore-endoplasmic reticulum contacts during the lipid transfer phase of mitophagy.

    Recommended Products

    Isotype Control

    Recommended Dilution Buffer

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    Rabbit Monoclonal Antibody Products IgG Antibody Products
    CAT Product Name Application Type
    MOR-0280 Hi-Affi™ Rabbit Anti-ATG4A Recombinant Antibody (clone DS280AB) IHC-P, IP, WB Rabbit IgG

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