Rabbit Anti-ATG4A Recombinant Antibody (clone R07-3J5)
CAT#: VS3-XY3270
It is a recombinant rabbit antibody targeting the Autophagy related 4A cysteine peptidase (ATG4A), which catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy. It reacts with human, mouse and rat ATG4A. It has been determined using Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64759/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_4_6.jpg
Normal Tissue
Figure 8 Skeletal muscle
Myocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_B_3_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36374/75174_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HUVEC TERT2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101844-ATG4A
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ATG4A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-3J5
- Applications
- WB, IHC-P, IP
- Related Disease
- Neurodegeneration With Brain Iron Accumulation
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IP: 1/20
Target
- Alternative Names
- APG4A; AUTL2; HsAPG4A
- Gene ID
- 115201
- UniProt ID
- Q8WYN0
- Long Name
- Autophagy related 4A cysteine peptidase
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q8WYN0)
Modification sites at neXtProt (NX_Q8WYN0)
- Protein Refseq
- XP_047297757.1
- Function
- Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.
Involved in phagophore growth during mitophagy independently of its protease activity and of ATG8 proteins: acts by regulating ATG9A trafficking to mitochondria and promoting phagophore-endoplasmic reticulum contacts during the lipid transfer phase of mitophagy.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Secondary Antibody
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See other products for "ATG4A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0280 | Hi-Affi™ Rabbit Anti-ATG4A Recombinant Antibody (clone DS280AB) | IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX73 | Mouse Anti-ATG4A Recombinant Antibody (clone 1458CT808.66.25.69) | WB, ICC, IHC, FC | Mouse IgG2b |
| VS3-WK809 | Rabbit Anti-ATG4A Recombinant Antibody (clone VS3-WK809) | IHC, IF, ELISA | Rabbit IgG |
| VS7-HM155 | Mouse Anti-ATG4A Recombinant Antibody (clone 8C9C2) | ICC, FCM | Mouse IgG2b |
| VS7-HM156 | Mouse Anti-ATG4A Recombinant Antibody (clone 8E8G2) | FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY589 | Anti-ATG4A Immunohistochemistry Kit | IHC |
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