AbPlus™ Anti-ATG5 Magnetic Beads (3C7)
CAT#: VS-0424-XY23
The AbPlus Anti-ATG5 Magnetic Beads (3C7) is an innovative affinity resin which is bound with anti-ATG5 specific antibody (3C7). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of ATG5 protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42973/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_A_9_8.jpg
Normal Tissue
Figure 4 Thyroid gland
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_B_2_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000057663-ATG5
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magrose bead (> 50 μmol/mL gel)
- Bead Ligand
- Anti-ATG5 specific antibody (3C7)
- Target
- ATG5
- Immunogen
- Recombinant ATG5 protein
- Target Species
- Human, Mouse, Rat
- Antibody Clone
- 3C7
- Bead Capacity
- 20-30 mg/mL binding antibody
- Bead size
- 10-37 μm
- Stability
- pH 2-14
- Format
- 20% Suspension
- Buffer
- PBS, pH 7.4, with 1% BSA
- Preservative
- 0.03% Proclin 300
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.
- Alternative Names
- Autophagy Related 5; Apoptosis-Specific Protein; APG5-LIKE; APG5L; ASP; ATG5 Autophagy Related 5 Homolog (S. Cerevisiae); APG5 (Autophagy 5, S. Cerevisiae)-Like; APG5 Autophagy 5-Like (S. Cerevisiae)
- Gene ID
- 9474
- UniProt ID
- Q9H1Y0
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ATG5"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0058MZ | Chicken Anti-ATG5 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
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| MOR-0283 | Hi-Affi™ Rabbit Anti-ATG5 Recombinant Antibody (clone DS283AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MRO-0137-CN | Rabbit Anti-ATG5 Recombinant Antibody (clone CBACN-051) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0038J | Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) | IF, ICC, WB, IHC-P, IP | Mouse IgG |
| ZG-0039J | Mouse Anti-ATG5 Recombinant Antibody (clone 8B4) | IHC-P, IF | Mouse IgG |
| VS3-WK1145 | Mouse Anti-ATG5 Recombinant Antibody (VS3-WK1145) | WB | Mouse IgG1, κ |
| VS3-WK1146 | Rabbit Anti-ATG5 Recombinant Antibody (VS3-WK1146) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC76 | CytoStream™ Rabbit Anti-ATG5 Recombinant Antibody (VS13-YC76) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY591 | Anti-ATG5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY592 | Anti-Mouse ATG5 Immunohistochemistry Kit | IHC |
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