Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) (CAT#: ZG-0038J)
This product is a mouse antibody that recognizes ATG5.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
IF
Figure 1 Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) in IF
Immunofluorescence analysis of Hela cell. 1, CREB-1 Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). 1.ATG5 mouse Monoclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min)
IHC
Figure 2 Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) in IHC
Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1.ATG5 Mouse Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IHC
Figure 3 Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1.ATG5 Mouse Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
WB
Figure 4 Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) in WB
Western blot analysis of Hela Cell Lysate using ATG5 Mouse mAb diluted at 1:10000
Specifications
- Immunogen
- Recombinant Protein of ATG5
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ATG5
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 3C7
- Applications
- IF/ICC, WB, IHC-P, IP
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation.
Target
- Alternative Names
- Autophagy Related 5; Apoptosis-Specific Protein; APG5-LIKE; APG5L; ASP; ATG5 Autophagy Related 5 Homolog (S. Cerevisiae); APG5 (Autophagy 5, S. Cerevisiae)-Like; APG5 Autophagy 5-Like (S. Cerevisiae)
- Gene ID
- 9474
- UniProt ID
- Q9H1Y0
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Customer Reviews and Q&As
Customer Review
Q&As
Perpetual Excellence
Because we have consistently ordered this antibody throughout multiple batches, we are confident that it will perform as expected in investigations.
Robust Function
Because it performs well in a variety of experimental settings, this antibody is a good option for studies.
Not in a Determinative Entirety
Our results were much more precise because our Western blots showed no non-specific binding.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# ZG-0038J, RRID: AB_3112060)
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For Research Use Only. Not For Clinical Use.
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