AbPlus™ Anti-ATP6V1D Magnetic Beads (VS-0724-YC573)
CAT#: VS-0724-YC573
The AbPlus Anti-ATP6V1D Magnetic Beads (VS-0724-YC573) is an innovative affinity resin which is bound with anti-ATP6V1D specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of ATP6V1D protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57316/1001_A5_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31515/120209_B_9_5.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31515/120209_A_8_5.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100554-ATP6V1D
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-ATP6V1D specific antibody
- Target
- ATP6V1D
- Immunogen
- Recombinant human ATP6V1D/Atp6m fragment derived from E. coli.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein. [provided by RefSeq, Jul 2008]
- Alternative Names
- ATPase H+ Transporting V1 Subunit D; ATPase, H+ Transporting, Lysosomal (Vacuolar Proton Pump); ATPase, H+ Transporting, Lysosomal 34kDa, V1 Subunit D; V-ATPase 28 KDa Accessory Protein; Vacuolar Proton Pump Subunit D; V-ATPase Subunit D; ATP6M; VATD; H(+)-Transporting Two-Sector ATPase, Subunit M; ATPase, H+ Transporting Lysosomal, Member M;
- Gene ID
- 51382
- UniProt ID
- Q9Y5K8
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Downloadable Resources
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ATP6V1D"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0492MZ | Mouse Anti-ATP6V1D Recombinant Antibody (clone CBLS-052) | WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0299 | Hi-Affi™ Rabbit Anti-ATP6V1D Recombinant Antibody (clone DS299AB) | WB, IHC-P, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY639 | Anti-ATP6V1D Immunohistochemistry Kit | IHC |
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