AbPlus™ Anti-ATP6V1E1 Magnetic Beads (VS-0724-YC952) (CAT#: VS-0724-YC952)
The AbPlus Anti-ATP6V1E1 Magnetic Beads (VS-0724-YC952) is an innovative affinity resin which is bound with anti-ATP6V1E1 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of ATP6V1E1 protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29196/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29196/if_selected.jpg

Figure 2 Pancreas
(Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18699/43010_A_3_3.jpg
(Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18699/43010_A_3_3.jpg

Figure 3 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18699/43010_A_8_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18699/43010_A_8_5.jpg

Figure 4 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29196/103291_A_5_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29196/103291_A_5_6.jpg

Figure 5 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18699/42743_B_9_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18699/42743_B_9_5.jpg

Figure 6 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18699/43010_A_9_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18699/43010_A_9_3.jpg

Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131100-ATP6V1E1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131100-ATP6V1E1
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-ATP6V1E1 specific antibody
- Target
- ATP6V1E1
- Immunogen
- Recombinant human ATP6V1E1 fragment derived from E. coli.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008]
- Alternative Names
- ATPase H+ Transporting V1 Subunit E1; ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E1; Vacuolar Proton Pump Subunit E 1; V-ATPase 31 KDa Subunit; V-ATPase Subunit E 1; ATP6E2; ATP6E; P31; ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E Isoform 1;
- Gene ID
- 529
- UniProt ID
- P36543
Related Resources
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