AbPlus™ Anti-ATP6V1E1 Magnetic Beads (VS-0724-YC952)

CAT#: VS-0724-YC952

The AbPlus Anti-ATP6V1E1 Magnetic Beads (VS-0724-YC952) is an innovative affinity resin which is bound with anti-ATP6V1E1 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of ATP6V1E1 protein under native and denaturing conditions.

Gene Expression
Figure 1 IF staining of human cell line A-431 Figure 2 Pancreas Figure 3 Kidney Figure 4 Testis Figure 5 Cerebral cortex Figure 6 Colon Figure 7 RNA cell line category: Low cell line specificity

Specifications

  • Applications
  • Immunoprecipitation, Protein Purification
  • Matrix
  • Magnetic bead
  • Bead Ligand
  • Anti-ATP6V1E1 specific antibody
  • Target
  • ATP6V1E1
  • Immunogen
  • Recombinant human ATP6V1E1 fragment derived from E. coli.
  • Target Species
  • Human
  • Bead Capacity
  • 40 mg/mL
  • Bead size
  • 25 μm
  • Format
  • Suspension
  • Concentration
  • 2 mg/mL
  • Buffer
  • PBS, pH 7.4
  • Preservative
  • 0.1% Sodium azide
  • Storage
  • Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.

Applications

  • Application Notes
  • The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.

Target

  • Introduction
  • This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008]
  • Alternative Names
  • ATPase H+ Transporting V1 Subunit E1; ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E1; Vacuolar Proton Pump Subunit E 1; V-ATPase 31 KDa Subunit; V-ATPase Subunit E 1; ATP6E2; ATP6E; P31; ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E Isoform 1;
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