Human Anti-B cells Recombinant Antibody (clone BB11) (CAT#: FAMAB-0090-CN)

Figure 1 Supernatants from the hybridoma clones were analyzed by flow cytometry

Peripheral blood leukocytes, granulocytes, HMY, and JURKA T cells were stained with human Mabs 50.2, 54.3, and B11, followed by FITC-conjugated anti-human IgM (open tracing) or with secondary antibody (filled tracing, control cells).

Magadán, S., Valladares, M., Suarez, E., Sanjuán, I., Molina, A., Ayling, C., ... & González-Fernández, A. (2002). Production of antigen-specific human monoclonal antibodies: comparison of mice carrying IgH/κ or IgH/κ/λ transloci. Biotechniques, 33(3), 680-690.

Figure 2 Microlymphocy-totoxicity assay.

DAUDI cells were incubated with MAb B11 and rabbit complement (B11 + rabbit C') or human serum (B11 + human C').Heat-inactivated human serum (B11 + inactivated C') or complement without relevant MAb (C') were used as controls. Eosin staining visualized the dead cells, which appeared as black dots.

Magadán, S., Valladares, M., Suarez, E., Sanjuán, I., Molina, A., Ayling, C., ... & González-Fernández, A. (2002). Production of antigen-specific human monoclonal antibodies: comparison of mice carrying IgH/κ or IgH/κ/λ transloci. Biotechniques, 33(3), 680-690.