Rabbit Anti-BACE1 Recombinant Antibody (clone R05-1D6)
CAT#: VS3-XY3272
It is a recombinant rabbit antibody specific for BACE1 (Beta-secretase 1), which is responsible for the proteolytic processing of the amyloid precursor protein (APP). It specifically reacts with human, mouse and rat BACE1. This product has been reported for use in Western Blot and Immunoprecipitation. The recommended dilutions of Western Blot and Immunoprecipitation is 1/500-1/1000 and 1/20.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/952_G12_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows cytoplasmic positivity.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_1_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/947_G12_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_B_7_5.jpg
Normal Tissue
Figure 5 Hippocampus
Glial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_B_8_6.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_5_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_7_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (fHDF/TERT166)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186318-BACE1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat BACE1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-1D6
- Applications
- WB, IP
- Related Disease
- Alzheimer Disease; Myositis
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IP: 1/20
Target
- Alternative Names
- ASP2; BACE; HSPC104
- Gene ID
- 23621
- UniProt ID
- P56817
- Long Name
- Beta-secretase 1
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome
- Post Translation Modifications
- N-Glycosylated.
Addition of a bisecting N-acetylglucosamine by MGAT3 blocks lysosomal targeting, further degradation and is required for maintaining stability under stress conditions.
Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein.3 Publications
Palmitoylation mediates lipid raft localization.
- Protein Refseq
- NP_001193978.1; NP_001193977.1
- Function
- Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Cleaves CHL1.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-405 | Human Anti-BACE1 Recombinant Antibody; scFv Fragment (PSBL-405) | ELISA, Block | Human scFv |
| HPAB-0285-YC-S(P) | Mouse Anti-BACE1 Recombinant Antibody; scFv Fragment (HPAB-0285-YC-S(P)) | ELISA, FuncS | Mouse scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1153CL-S(P) | Mouse Anti-BACE1 Recombinant Antibody; scFv Fragment (TAB-1153CL-S(P)) | ELISA | Mouse scFv |
| TAB-1154CL-S(P) | Mouse Anti-BACE1 Recombinant Antibody; scFv Fragment (TAB-1154CL-S(P)) | WB, ELISA | Mouse scFv |
| TAB-1155CL-S(P) | Mouse Anti-BACE1 Recombinant Antibody; scFv Fragment (TAB-1155CL-S(P)) | ELISA, Inhib, FuncS | Mouse scFv |
| TAB-1156CL-S(P) | Mouse Anti-BACE1 Recombinant Antibody; scFv Fragment (TAB-1156CL-S(P)) | ELISA, Inhib, FuncS | Mouse scFv |
| TAB-1153CL-F(E) | Mouse Anti-BACE1 Recombinant Antibody; Fab Fragment (TAB-1153CL-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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