Mouse Anti-Bet v 1 Recombinant Antibody (clone BV16) (CAT#: HPAB-S0006-YC)
BV16 is a murine IgG1 antibody raised by immunization with purified Bet v 1. BV16 partially inhibits the binding of human serum IgE to Bet v 1, the epitope defined by BV16 is partly or completely overlapping Bet v 1 epitopes recognized by human serum IgE originating from natural exposure.
Specific Inquiry
ELISA
Figure 1 Inhibition of IgE binding to surface-coated △29NCS_5x in the presence of both Bet v 1 and serial dilutions of Bet v 1-binding monoclonal mouse IgG antibody BV16 in ELISA.
In the absence of BV16, IgE binding to △29NCS_5x was inhibited to 84% by Bet v 1. The observed Bet v 1-induced inhibition of IgE-△29NCS_5x interaction could, however, been abolished by the monoclonal antibody BV16 in a dose-dependent manner. This result suggests a structural overlap between the individual cross-reactive IgE epitope of △29NCS_5x and the Bet v1-specific IgG epitope recognized by monoclonal antibody BV16.
Berkner, H., Seutter von Loetzen, C., Hartl, M., Randow, S., Gubesch, M., Vogel, L., ... & Schiller, D. (2014). Enlarging the toolbox for allergen epitope definition with an allergen-type model protein. PloS one, 9(10), e111691.
ELISA
Figure 2 Binding of serial dilutions of monoclonal BV16 to surface coated equimolar amounts of Bet v 1 and Bet v 1_4x in ELISA.
Interaction of monoclonal BV16 with Bet v 1_4x was virtually abolished.
Berkner, H., Seutter von Loetzen, C., Hartl, M., Randow, S., Gubesch, M., Vogel, L., ... & Schiller, D. (2014). Enlarging the toolbox for allergen epitope definition with an allergen-type model protein. PloS one, 9(10), e111691.
Specifications
- Immunogen
- Purified Bet v 1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Species Reactivity
- Birch
- Clone
- BV16
- Applications
- ELISA, IB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Birch pollen allergen Bet v1; Bet v1
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1964-FY | Human Anti-Bet v 1 Recombinant Antibody (clone BAB2) | WB, ELISA | Human IgG |
| HPAB-1965-FY | Human Anti-Bet v 1 Recombinant Antibody (clone BAB3) | WB, ELISA | Human IgG |
| HPAB-1966-FY | Human Anti-Bet v 1 Recombinant Antibody (clone BAB4) | WB, ELISA | Human IgG |
| FAMAB-1841CQ | Rabbit Anti-Bet v 1 Recombinant Antibody (clone BAB5) | WB, ELISA | Rabbit IgG |
| VS-0322-LC58 | Rabbit Anti-Bet v 1 Recombinant Antibody (clone BAB3) | WB, ELISA |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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