Human Anti-Biotin Recombinant Antibody (HPAB-0109-YJ) (CAT#: HPAB-0109-YJ)
This product is a recombinant human antibody that recognizes Biotin. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Specific Inquiry
WB
Figure 1 Recombinant Humanized Anti-Biotin Antibody (HPAB-0109-YJ) in WB
Western blot analysis of HPAB-0109-YJ was performed with Biotin (His Tag).
Lane 1: Reducing antigen (0.2μg)
Lane 2: Reducing antigen (0.4μg)
Lane 3: Reducing antigen (0.8μg)
The secondary antibody: HRP Goat Anti-Human IgG (H+L)
ELISA
Figure 2 Recombinant Humanized Anti-Biotin Antibody (HPAB-0109-YJ) in ELISA
ELISA analysis of HPAB-0109-YJ was performed by coating with biotin (His Tag).
The secondary antibody: HRP Goat Anti-Human IgG (H+L)
SDS-PAGE
Figure 3 Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in SDS-PAGE
SDS-PAGE analysis of HPAB-0109-YJ in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of HPAB-0109-YJ was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 4 Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in HPLC
The purity of HPAB-0109-YJ was greater than 95% as determined by SEC-HPLC.
WB
Figure 5 Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in WB
Western blot analysis of HPAB-0109-YJ was performed by loading recombinant protein with biotin labeled.
HPAB-0109-YJ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 6 Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in ELISA
ELISA analysis of HPAB-0109-YJ was performed by coating with recombinant protein with biotin labeled.
The secondary antibody: HRP-goat anti-human IgG
SDS-PAGE
Figure 7 Afuco™ Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in SDS-PAGE
SDS-PAGE analysis of Afucosylated HPAB-0109-YJ in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of Afucosylated HPAB-0109-YJ was greater than 95% as determined by SDS-PAGE.
WB
Figure 8 Afuco™ Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in WB
Western blot analysis of Afucosylated HPAB-0109-YJ was performed by loading a antibody with biotin conjugated.
Afucosylated HPAB-0109-YJ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 9 Afuco™ Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in ELISA
ELISA analysis of Afucosylated HPAB-0109-YJ was performed by coating with a antibody with biotin conjugated. Then blocked with BSA, and incubated with Afucosylated anti-Biotin recombinant antibody (clone huM33) (HPAB-0109-YJ). The goat anti-human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG1, κ
- Specificity
- Chemical
- Species Reactivity
- Chemical
- Applications
- SPR
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Biotin
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0107-YJ | Mouse Anti-Biotin Recombinant Antibody (HPAB-0107-YJ) | SPR | Mouse IgG1, κ |
| HPAB-S0187-YC | Mouse Anti-Biotin Recombinant Antibody (clone M33) | ELISA | Mouse IgG |
| FAMAB-1598CQ | Mouse Anti-Biotin Recombinant Antibody (clone BK-1/39) | ChIP, IP, FC, IHC | Mouse IgG1 |
| 2RD-A1556-YJ | HRP-conjugated Streptavidin | ELISA, WB | |
| VS-0224-XY14 | Rabbit Anti-Biotin Recombinant Antibody (clone chiBK-1/39) | FC, IP, IHC, ChIP | Rabbit IgG, kappa |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0109-YJ, RRID: AB_3111356)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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