Rabbit Anti-BRAF (V600E) Recombinant Antibody (clone B16-U) (CAT#: ZG-0301U)

Rabbit anti-BRAF (V600E) antibody (clone B16-U) displayed high specificity against the BRAF (V600E) protein of Human, mouse and rat.

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IHC-P

Figure 1 Rabbit Anti-BRAF (V600E) Antibody (ZG-0301U) in IHC-P

Figure 1 Rabbit Anti-BRAF (V600E) Antibody (ZG-0301U) in IHC-P

Result of the Leica BOND Max immunohistochemical staining protocol on a formalin fixed and paraffin embedded human melanoma tissues (4 μm sections) with Anti-BRAF V600-E (ZG-0301U) antibody. The fine granular brown staining in the cytoplasm of the tumor cells, highlights specifically V600-E mutation of BRAF protein, while dark brown dots represent melanin pigment deposition.


Specifications

  • Immunogen
  • Peptide corresponding to V600-E mutation of human BRAF (V637-E of mouse or rat BRAF)
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human BRAF (V600E)
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • B16-U
  • Applications
  • IHC-P
  • Conjugate
  • Unconjugated
  • Related Disease
  • Cardiofaciocutaneous Syndrome 1 and Lung Cancer.

Product Property

  • Purification
  • Affinity Purified
  • Buffer
  • 20 mM Tris-HCl, pH 8.0, 20 mg/ml BSA and 0.05% sodium azide
  • Preservative
  • 0.05% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Immunohistochemistry-Paraffin. Specific conditions should be optimized by the end user.
    IHC-P: 1:100-1:200

Target

  • Alternative Names
  • NS7; B-raf; BRAF1; RAFB1; B-RAF1; serine/threonine-protein kinase B-raf; 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; murine sarcoma viral (v-raf) oncogene homolog B1; proto-oncogene B-Raf; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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