Human Anti-C. botulinum BoNT/B Recombinant Antibody (clone MCS6-27) (CAT#: FAMAB-1596CQ)

Figure 1 Antibody binding on Western blots of reduced and nonreduced BoNT/B.

BoNT/B was separated on 10% SDS-PAGE in the presence (+) and absence (-) of 10 mM DTT, transferred to nitrocellulose membranes and probed with mAbs F24-1, F26-16, F27- 33, F29-40 and MCS6-27. Bands showing the intact holotoxin (Holo.), heavy chain (Hc) and light chain (Lc) are indicated. Protein relative molecular weight (rMW) is indicated in kDa.

Scotcher, M. C., Cheng, L. W., & Stanker, L. H. (2010). Detection of botulinum neurotoxin serotype B at sub mouse LD50 levels by a sandwich immunoassay and its application to toxin detection in milk. PLoS One, 5(6), e11047.

Figure 2 Antibody binding to BoNT serotypes by direct binding ELISA.

Antibody binding to black microtiter plates coated with 0.1 ug/mL solutions of BoNT serotypes A through G was measured. Data are presented as average of 3 samples ±1 standard error.

Scotcher, M. C., Cheng, L. W., & Stanker, L. H. (2010). Detection of botulinum neurotoxin serotype B at sub mouse LD50 levels by a sandwich immunoassay and its application to toxin detection in milk. PLoS One, 5(6), e11047.

Figure 3 Sandwich ELISA for detection of BoNT/B.

MAb MCS6-27 was used as capture antibody, rabbit anti-BoNT/B polyclonal was used in conjunction with an HRP-conjugated, goat anti-rabbit polyclonal antibody as detector. Data are presented ±1 standard error, n = 3.
Horizontal dashed lines represent the limit of detection (L.O.D., zero toxin plus 3 standard deviations) and the limit of quantitation (L.O.Q., zero toxin plus 5 standard deviations).

Scotcher, M. C., Cheng, L. W., & Stanker, L. H. (2010). Detection of botulinum neurotoxin serotype B at sub mouse LD50 levels by a sandwich immunoassay and its application to toxin detection in milk. PLoS One, 5(6), e11047.