Rabbit Anti-CALU Recombinant Antibody (clone R05-1E7)
CAT#: VS3-FY1981
Clone R05-1E7 is a rabbit recombinant monoclonal antibody that attaches to human CALU. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-P. This calumenin antibody was used in western blot analysis of calumenin in Hela, A549, C6 lysates and immunohistochemistry analysis of paraffin-embedded human colon cancer.
Tested Data
Gene Expression
WB
Figure 1 Anti-CALU Antibody (VS3-FY1981) in WB.
The Calumenin expression in Hela, A549, C6 lysates were detected using the anti-Calumenin by western blot assay.
IHC
Figure 2 Anti-CALU Antibody (VS3-FY1981) in IHC.
Immunohistochemistry staining of paraffin-embedded human colon cancer using the anti-Calumenin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6018/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6018/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6018/18212_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6018/18212_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6018/18212_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6018/18212_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6018/18212_A_6_6.jpg
Normal Tissue
Figure 8 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6018/18212_A_2_7.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6018/18212_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (fHDF/TERT166)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128595-CALU
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Specifications
- Immunogen
- Synthetic peptide of human Calumenin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CALU
- Species Reactivity
- Human, Rat
- Clone
- R05-1E7
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 37 kDa, while the observed molecular weight stands at 47 kDa.
- Related Disease
- Human colon cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Calumenin; IEF SSP 9302; Multiple EF-Hand Protein; Crocalbin-Like Protein; Crocalbin
- Gene ID
- 813
- UniProt ID
- O43852
- Sequence Similarities
- Belongs to the CREC family.
- Cellular Localization
- Endoplasmic reticulum, Golgi apparatus, Membrane, Sarcoplasmic reticulum, Secreted
- Post Translation Modifications
- Glycosylation at Thr60, Ser261, and Asn131 (NX_O43852 [NX_O43852-1])
Ubiquitination at Lys84, Lys94, Lys98, Lys255, and Lys114 (NX_O43852 [NX_O43852-1])
Modification sites at PhosphoSitePlus (O43852)
Glycosylation from GlyConnect CALU_HUMAN (1062)
Glycosylation from GlyGen (O43852) 9 sites, 45 N-linked glycans (1 site), 3 O-linked glycans (8 sites)
- Protein Refseq
- NP_001124146.1; NP_001186600.1; NP_001186601.1; NP_001186602.1; NP_001210.1
- Function
- Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CALU"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3224z | Mouse Anti-CALU Recombinant Antibody (clone 39B12) | WB, IP, IF, IHC, ELISA | Mouse IgG2a, κ |
| ZG-0360F | Mouse Anti-CALU Recombinant Antibody (ZG-0360F) | WB | Mouse IgG |
| VS4-WK55 | Mouse Anti-CALU Recombinant Antibody (VS4-WK55) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0444 | Hi-Affi™ Rabbit Anti-CALU Recombinant Antibody (clone DS444AB) | WB, IHC, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY298 | Anti-CALU Immunohistochemistry Kit | IHC |
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