Rabbit Anti-CAP2 Recombinant Antibody (clone R04-3D1)
CAT#: VS3-FY1955
It is a amazing rabbit recombinant monoclonal antibody that was purified by affinity chromatography. It specifically binds to human CAP2. It is suitable for use in a variety of assays, including WB, IP. The following concentration ranges are recommended starting points for this product: western blot (dilution 1/500-1/1000), immunoprecipitation (dilution 1/20).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50530/if_selected.jpg
Normal Tissue
Figure 2 High expression in cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50530/123823_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50530/123823_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Distal tubules Staining: Low Intensity: Weak Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50530/123823_A_8_5.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50530/123823_B_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (LHCN-M2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112186-CAP2
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WB
Figure 1 Anti-CAP2 Antibody (VS3-FY1955) in WB.
The CAP2 expression in Hela, CHO-K1 lysates were detected using the anti-CAP2 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human CAP2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CAP2
- Species Reactivity
- Human
- Clone
- R04-3D1
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 53 kDa, while the observed molecular weight stands at 53 kDa.
- Related Disease
- Cardiomyopathy, Dilated, 2I and Familial Isolated Dilated Cardiomyopathy
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cyclase Associated Actin Cytoskeleton Regulatory Protein 2; CAP 2; CAP, Adenylate Cyclase-Associated Protein, 2 (Yeast); CAP, Adenylate Cyclase-Associated Protein, 2; Adenylyl Cyclase-Associated Protein 2; 2810452G09Rik
- Gene ID
- 10486
- UniProt ID
- P40123
- Sequence Similarities
- Belongs to the CAP family.
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P40123)
Modification sites at neXtProt (NX_P40123)
Glycosylation from GlyGen (P40123) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001350462.1; NP_001350463.1; NP_006357.1
- Function
- May have a regulatory bifunctional role.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CAP2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0458 | Hi-Affi™ Rabbit Anti-CAP2 Recombinant Antibody (clone DS458AB) | WB, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY306 | Anti-CAP2 Immunohistochemistry Kit | IHC |
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