Rabbit Anti-CARS Recombinant Antibody (clone R01-7D4) (CAT#: VS3-FY1982)
The R01-7D4 recombinant monoclonal antibody detects human CARS. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-P. This CARS antibody was verified by western blot analysis of CARS in Hela, A549, HL-60, U2OS, C6 lysates and immunohistochemistry analysis of paraffin-embedded human breast cancer.
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WB
Figure 1 Anti-CARS Antibody (VS3-FY1982) in WB.
The CARS expression in Hela, A549, HL-60, U2OS, C6 lysates were detected using the anti-CARS by western blot assay.
IHC
Figure 2 Anti-CARS Antibody (VS3-FY1982) in IHC.
Immunohistochemistry staining of paraffin-embedded human breast cancer using the anti-CARS antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptides for human CARS
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CARS
- Species Reactivity
- Human, Rat
- Clone
- R01-7D4
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 85 kDa, while the observed molecular weight stands at 85 kDa.
- Related Disease
- Human breast cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Cysteinyl-TRNA Synthetase; Cysteine TRNA Ligase 1, Cytoplasmic; EC 6.1.1.16; CYSRS; Cysteine--TRNA Ligase, Cytoplasmic; Cysteine Translase; MGC:11246; EC 6.1.1; CARS1
- Gene ID
- 833
- UniProt ID
- P49589
- Sequence Similarities
- Belongs to the class-I aminoacyl-tRNA synthetase family.
- Cellular Localization
- Cytoplasm
- Function
- Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0469 | Hi-Affi™ Rabbit Anti-CARS Recombinant Antibody (clone DS469AB) | WB, IHC, ICC, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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