Rabbit Anti-CBX4 Recombinant Antibody (clone R08-4I3)
CAT#: VS3-FY1984
The R08-4I3 recombinant monoclonal antibody detects human CBX4. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IP. Applications Tested: The CBX4 antibody has been tested by western blot analysis of CBX4 in 3T3 lysates and western blot analysis of CBX4 in Hela, A549, HL-60, U2OS, C6 lysates.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in subset of hematopoietic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8228/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12021/1279_H2_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12021/912_E10_1_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8228/21192_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8228/21192_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8228/21192_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8228/21192_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (SK-BR-3, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141582-CBX4
❮
❯
WB
Figure 1 Anti-CBX4 Antibody (VS3-FY1984) in WB.
The CBX4 expression in 3T3 lysates were detected using the anti-CBX4 by western blot assay.
WB
Figure 2 Anti-CBX4 Antibody (VS3-FY1984) in WB.
The CBX4 expression in Hela, A549, HL-60, U2OS, C6 lysates were detected using the anti-CBX4 by western blot assay.
❮
❯
Specifications
- Immunogen
- Synthetic peptide of human CBX4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CBX4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-4I3
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 61 kDa, while the observed molecular weight stands at 61 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Chromobox 4; Chromobox Protein Homolog 4; NS5ATP1-Binding Protein 16; Polycomb 2 Homolog; PC2; Chromobox Homolog 4 (Pc Class Homolog, Drosophila); Chromobox Homolog 4 (Drosophila Pc Class); Pc Class 2 Homolog (Drosophila)
- Gene ID
- 8535
- UniProt ID
- O00257
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitinated.
Ubiquitination regulates the function of the Polycomb group (PcG) multiprotein PRC1-like complex.
Deubiquitinated by USP26.
Phosphorylated on Thr-497 by HIPK2 upon DNA damage.
This phosphorylation stimulates E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494, as well as sumoylation of other target proteins, such as HNRNPK.
Modification sites at PhosphoSitePlus (O00257)
Modification sites at neXtProt (NX_O00257)
- Protein Refseq
- XP_011523701.1; XP_054173585.1; NP_003646.2
- Function
- E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I.
Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY1984. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CBX4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-272 | Human Anti-CBX4 Recombinant Antibody (clone D395) | ELISA, IP-MS | Human IgG1 |
| VS3-XY172 | Mouse Anti-CBX4 Recombinant Antibody (clone 2A1H5E1) | ELISA, WB, FC, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0497 | Hi-Affi™ Rabbit Anti-CBX4 Recombinant Antibody (clone DS497AB) | FC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1039 | Anti-CBX4 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: Inhib, Cyt
-2.png)
Application: IB, ELISA, FC, FuncS
Application: FC, FRET, Internalization
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FC, Inhib
Application: FC, IHC, Cyt, FuncS
Application: WB, FC, IF, Inhib, ELISA, IHC
-2.png)
Application: ELISA
Application: ELISA, Cyt, PP, Inhib
-3.jpg)
Application: IB, FC, ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
