Rabbit Anti-CBX4 Recombinant Antibody (clone R08-4I3) (CAT#: VS3-FY1984)
The R08-4I3 recombinant monoclonal antibody detects human CBX4. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IP. Applications Tested: The CBX4 antibody has been tested by western blot analysis of CBX4 in 3T3 lysates and western blot analysis of CBX4 in Hela, A549, HL-60, U2OS, C6 lysates.
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WB
Figure 1 Anti-CBX4 Antibody (VS3-FY1984) in WB.
The CBX4 expression in 3T3 lysates were detected using the anti-CBX4 by western blot assay.
WB
Figure 2 Anti-CBX4 Antibody (VS3-FY1984) in WB.
The CBX4 expression in Hela, A549, HL-60, U2OS, C6 lysates were detected using the anti-CBX4 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human CBX4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CBX4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-4I3
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 61 kDa, while the observed molecular weight stands at 61 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Chromobox 4; Chromobox Protein Homolog 4; NS5ATP1-Binding Protein 16; Polycomb 2 Homolog; PC2; Chromobox Homolog 4 (Pc Class Homolog, Drosophila); Chromobox Homolog 4 (Drosophila Pc Class); Pc Class 2 Homolog (Drosophila)
- Gene ID
- 8535
- UniProt ID
- O00257
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitinated.
Ubiquitination regulates the function of the Polycomb group (PcG) multiprotein PRC1-like complex.
Deubiquitinated by USP26.
Phosphorylated on Thr-497 by HIPK2 upon DNA damage.
This phosphorylation stimulates E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494, as well as sumoylation of other target proteins, such as HNRNPK.
Modification sites at PhosphoSitePlus (O00257)
Modification sites at neXtProt (NX_O00257)
- Protein Refseq
- XP_011523701.1; XP_054173585.1; NP_003646.2
- Function
- E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I.
Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-272 | Human Anti-CBX4 Recombinant Antibody (clone D395) | ELISA, IP-MS | Human IgG1 |
| VS3-XY172 | Mouse Anti-CBX4 Recombinant Antibody (clone 2A1H5E1) | ELISA, WB, FC, IHC | Mouse IgG1 |
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