Rabbit Anti-CBX4 Recombinant Antibody (clone R08-4I3) (CAT#: VS3-FY1984)
The R08-4I3 recombinant monoclonal antibody detects human CBX4. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IP. Applications Tested: The CBX4 antibody has been tested by western blot analysis of CBX4 in 3T3 lysates and western blot analysis of CBX4 in Hela, A549, HL-60, U2OS, C6 lysates.
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WB
Figure 1 Anti-CBX4 Antibody (VS3-FY1984) in WB.
The CBX4 expression in 3T3 lysates were detected using the anti-CBX4 by western blot assay.
WB
Figure 2 Anti-CBX4 Antibody (VS3-FY1984) in WB.
The CBX4 expression in Hela, A549, HL-60, U2OS, C6 lysates were detected using the anti-CBX4 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human CBX4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CBX4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-4I3
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 61 kDa, while the observed molecular weight stands at 61 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Chromobox 4; Chromobox Protein Homolog 4; NS5ATP1-Binding Protein 16; Polycomb 2 Homolog; PC2; Chromobox Homolog 4 (Pc Class Homolog, Drosophila); Chromobox Homolog 4 (Drosophila Pc Class); Pc Class 2 Homolog (Drosophila)
- Gene ID
- 8535
- UniProt ID
- O00257
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitinated.
Ubiquitination regulates the function of the Polycomb group (PcG) multiprotein PRC1-like complex.
Deubiquitinated by USP26.
Phosphorylated on Thr-497 by HIPK2 upon DNA damage.
This phosphorylation stimulates E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494, as well as sumoylation of other target proteins, such as HNRNPK.
Modification sites at PhosphoSitePlus (O00257)
Modification sites at neXtProt (NX_O00257)
- Protein Refseq
- XP_011523701.1; XP_054173585.1; NP_003646.2
- Function
- E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I.
Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-272 | Human Anti-CBX4 Recombinant Antibody (clone D395) | ELISA, IP-MS | Human IgG1 |
| VS3-XY172 | Mouse Anti-CBX4 Recombinant Antibody (clone 2A1H5E1) | ELISA, WB, FC, IHC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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