Rabbit Anti-CD82 Recombinant Antibody (clone R06-7K5)
CAT#: VS3-XY3301
The product is a recombinant rabbit antibody against CD82 (CD82 molecule), which associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. It specifically reacts with human and rat CD82. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin and Flow cytometry.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61418/1143_C8_4_selected.jpg
Subcellular Location
Figure 2 IHC staining of human appendix
Immunohistochemical staining of human appendix shows strong positivity in lymphoid tissue.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_2_2_selected.jpg
Subcellular Location
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows distinct positivity of reticular fibers.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2508/ihc_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61418/1130_C8_1_blue_red_green.jpg
Subcellular Location
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61418/1242_A11_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_B_9_5.jpg
Normal Tissue
Figure 7 Pancreas
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_1_3.jpg
Normal Tissue
Figure 8 Kidney
Collecting ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_9_5.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HMC-1, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000085117-CD82
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CD82
- Species Reactivity
- Human, Rat
- Clone
- R06-7K5
- Applications
- WB, IHC-P, FC
- Related Disease
- Prostate Cancer; Bladder Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
FC: 1/50-1/100
Target
- Alternative Names
- R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
- Gene ID
- 3732
- UniProt ID
- P27701
- Long Name
- CD82 molecule
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Glycosylation at Asn129, Asn157, and Asn198 (NX_P27701 [NX_P27701-1])
Ubiquitination at Lys190 (NX_P27701 [NX_P27701-1])
Modification sites at PhosphoSitePlus (P27701)
- Protein Refseq
- XP_011518369.1; XP_047282856.1; XP_047282857.1; XP_047282859.1; XP_047282860.1
- Function
- Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- CAT
- Product Name
Recommended Dilution Buffer
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Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1231 | Anti-CD82 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1232 | Anti-Rat CD82 Immunohistochemistry Kit | IHC |
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