Rabbit Anti-CD82 Recombinant Antibody (clone R06-7K5) (CAT#: VS3-XY3301)
The product is a recombinant rabbit antibody against CD82 (CD82 molecule), which associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. It specifically reacts with human and rat CD82. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin and Flow cytometry.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61418/1143_C8_4_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61418/1143_C8_4_selected.jpg
Figure 2 IHC staining of human appendix
(Immunohistochemical staining of human appendix shows strong positivity in lymphoid tissue.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_2_2_selected.jpg
(Immunohistochemical staining of human appendix shows strong positivity in lymphoid tissue.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_2_2_selected.jpg
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows distinct positivity of reticular fibers.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2508/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows distinct positivity of reticular fibers.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2508/ihc_selected.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61418/1130_C8_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61418/1130_C8_1_blue_red_green.jpg
Figure 5 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61418/1242_A11_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61418/1242_A11_1_blue_red_green.jpg
Figure 6 Cerebral cortex
(Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_B_9_5.jpg
(Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_B_9_5.jpg
Figure 7 Pancreas
(Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_1_3.jpg
(Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_1_3.jpg
Figure 8 Kidney
(Collecting ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_9_5.jpg
(Collecting ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_9_5.jpg
Figure 9 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_7_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_7_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (HMC-1, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000085117-CD82
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000085117-CD82
Figure 10 RNA cell line category: Cell line enhanced (HMC-1, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000085117-CD82
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000085117-CD82
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CD82
- Species Reactivity
- Human, Rat
- Clone
- R06-7K5
- Applications
- WB, IHC-P, FC
- Related Disease
- Prostate Cancer; Bladder Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
FC: 1/50-1/100
Target
- Alternative Names
- R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
- Gene ID
- 3732
- UniProt ID
- P27701
- Long Name
- CD82 molecule
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Glycosylation at Asn129, Asn157, and Asn198 (NX_P27701 [NX_P27701-1])
Ubiquitination at Lys190 (NX_P27701 [NX_P27701-1])
Modification sites at PhosphoSitePlus (P27701)
- Protein Refseq
- XP_011518369.1; XP_047282856.1; XP_047282857.1; XP_047282859.1; XP_047282860.1
- Function
- Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1186z | Mouse Anti-CD82 Recombinant Antibody (clone 28B4) | WB, FC, ICC, IHC, IP, FuncS | Mouse IgG2a |
| MOB-1605MZ | Recombinant Mouse Anti-Human CD82 Antibody (clone C-M3), PE-Conjugated | FC | Mouse antibody |
| VS3-FY322 | Recombinant Mouse Anti-CD82 Antibody (clone 5D5D5) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY323 | Recombinant Mouse Anti-CD82 Antibody (clone 7G8G4) | ELISA, IHC, FC | Mouse IgG2a |
| VS13-YC178 | Rabbit Anti-CD82 Recombinant Antibody (VS13-YC178) | IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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