Human Anti-Ch14.18 Recombinant Antibody (clone GK8) (CAT#: FAMAB-0035JF)
Recombinant monoclonal antibody can sepecially bind to ch14.18. This human anti-ch4.18 antibody GK8 can be poteincially used in the treatment of patients with Neuroblastoma.
Specific Inquiry
WB
Figure 1 Human Anti-Ch14.18 Antibody (clone GK8) in WB.
Western blot analysis of purified GK2 and GK8 Fab and GK2 and GK8 Fab phage. a, SDS-Page of blotted, purified GK2 and GK8 Fabs, and pooled human Fabs as a control is shown.
Uttenreuther-Fischer, M. M., Krüger, J. A., & Fischer, P. (2006). Molecular characterization of the anti-idiotypic immune response of a relapse-free neuroblastoma patient following antibody therapy: a possible vaccine against tumors of neuroectodermal origin?. The Journal of Immunology, 176(12), 7775-7786.
Neut
Figure 2 Human Anti-Ch14.18 Antibody (clone GK8) in Neut.
Competition of purified Fabs with ch14.18 on GD2. GK2 and GK8 Fabs were purified by affinity chromatography using immobilized anti-human Fab Abs. Competition of purified Fabs with ch14.18 on GD2 clearly showed that GK2 Fab and GK8 Fab were able to significantly inhibit (GK2 Fab) or completely suppress binding (GK8 Fab) of ch14.18 to GD2 even at low concentrations, in contrast with an anti-BSA-Fab (negative control). Depicted is the percentage of binding inhibition as determined by B/Bo, where B is absorbance with inhibitor and Bo is absorbance without inhibitor. Bo was set at measurement end point OD of 2.5.
Uttenreuther-Fischer, M. M., Krüger, J. A., & Fischer, P. (2006). Molecular characterization of the anti-idiotypic immune response of a relapse-free neuroblastoma patient following antibody therapy: a possible vaccine against tumors of neuroectodermal origin?. The Journal of Immunology, 176(12), 7775-7786.
Neut
Figure 3 Human Anti-Ch14.18 Antibody (clone GK8) in Neut.
Competition of purified Fabs with the natural anti-idiotypic Abs of patient 1 on 14G2a. Purified GK2 and GK8 Fabs competed with the natural anti-idiotypic Abs of patient 1, i.e., diluted patient's serum, for binding to 14G2a. GK8 Fab demonstrated nearly complete binding inhibition, indicating that it represents the majority of the patient's anti-idiotypic Abs. The patient's serum had to be diluted to adjust for comparable IgG concentrations of both competitors.
Uttenreuther-Fischer, M. M., Krüger, J. A., & Fischer, P. (2006). Molecular characterization of the anti-idiotypic immune response of a relapse-free neuroblastoma patient following antibody therapy: a possible vaccine against tumors of neuroectodermal origin?. The Journal of Immunology, 176(12), 7775-7786.
Specifications
- Immunogen
- Ch14.18
- Host Species
- Human
- Derivation
- Human phage display Fab library
- Type
- Human IgG
- Specificity
- Ch14.18
- Clone
- GK8
- Applications
- WB, ELISA, FuncS
- Related Disease
- Neuroblastoma
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Chimeric monoclonal antibody ch14.18; ch14.18
- Function
- Inhibitor
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Anti-idiotypic Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0035JF-F(E) | Human Anti-Ch14.18 Recombinant Antibody (clone GK8); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| FAMAB-0035JF-S(P) | Human Anti-Ch14.18 Recombinant Antibody (clone GK8); scFv Fragment | WB, ELISA, FuncS | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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