Rabbit Anti-CLTC Recombinant Antibody (VS13-YC229)
CAT#: VS13-YC229
The Rabbit Anti-CLTC Recombinant Antibody is specific for human, mouse and rat CLTC. CLTC, a major clathrin component, influences vesicular trafficking and macromolecule endocytosis, enabling receptor sorting and organelle maintenance. The anti-CLTC antibody can be used for Western blot (1/500-1/2000), Immunohistochemistry-paraffin (1/50-1/200).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11571/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17155/623_C4_1_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59143/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human testis
Immunohistochemical staining of human testis shows cytoplasmic positivity in cells of seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10389/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human epididymis
Immunohistochemical staining of human epididymis shows distinct cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11571/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 6 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17155/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 7 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11571/641_F2_1_blue_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17155/38923_B_7_5.jpg
Normal Tissue
Figure 9 Parathyroid gland
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17155/38923_A_2_8.jpg
Normal Tissue
Figure 10 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17155/38923_A_7_3.jpg
Normal Tissue
Figure 11 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17155/38923_A_8_5.jpg
Normal Tissue
Figure 12 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17155/38923_A_6_6.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141367-CLTC
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Specifications
- Immunogen
- Recombinant human Clathrin heavy chain, aa 201-250.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CLTC
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Target
- Alternative Names
- Clathrin Heavy Chain; Clathrin Heavy Chain On Chromosome 17; Clathrin, Heavy Polypeptide-Like 2; Clathrin, Heavy Polypeptide (Hc); CLTCL2; CLH-17; Clathrin, Heavy Chain (Hc); Clathrin Heavy Chain 1;
- Gene ID
- 1213
- UniProt ID
- Q00610
- Long Name
- Clathrin Heavy Chain
- Cellular Localization
- Cytoplasmic vesicle; Cell membrane
- Function
- Signal transduction
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CLTC"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-674-VHH | Recombinant Anti-Human CLTC VHH Single Domain Antibody | FA, FC, ICC, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3292z | Mouse Anti-CLTC Recombinant Antibody (clone 38H7) | FC, IF, WB | Mouse IgG1, κ |
| MOB-1100MZ | Recombinant Mouse Anti-Human CLTC Antibody (clone 4G243) | WB | Mouse antibody |
| VS3-WK257 | Rabbit Anti-CLTC Recombinant Antibody (clone JA53-02) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-469LC | Mouse Anti-CLTC Recombinant Antibody (TAB-469LC) | ELISA, FC, FuncS, Inhib, WB | Mouse IgG |
| TAB-469LC-S(P) | Mouse Anti-CLTC Recombinant Antibody; scFv Fragment (TAB-469LC-S(P)) | WB, ELISA, IHC | Mouse scFv |
| TAB-469LC-F(E) | Mouse Anti-CLTC Recombinant Antibody; Fab Fragment (TAB-469LC-F(E)) | WB, ELISA, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-480CQ | Mouse Anti-CLTC Recombinant Antibody (clone X22) | Block, ELISA, FC, ICC, IF, IHC, IHC-P, IM, IP, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0734 | Hi-Affi™ Rabbit Anti-CLTC Recombinant Antibody (clone DS734AB) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY128 | Mouse Anti-NHP CLTC Recombinant Antibody (clone X22) | WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY479 | Anti-CLTC Immunohistochemistry Kit | IHC | |
| VS-0525-XY1532 | Anti-Mouse CLTC Immunohistochemistry Kit | IHC | |
| VS-0525-XY1531 | Anti-Human CLTC Immunohistochemistry Kit | IHC | |
| VS-0525-XY1533 | Anti-Bovine CLTC Immunohistochemistry Kit | IHC |
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