Recombinant Mouse Anti-CVA10 Antibody (PABC-309) (CAT#: PABC-309)

This product is a recombinant mouse antibody that can recognize CVA10. This antibody cross-reacts with three forms of capsids and binds at a border region spanning all three viral proteins, VP1 to VP3. It exhibits high neutralizing activity in the neutralization assay in vitro.

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  • Tested Data
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ELISA

Figure 1 Binding efficacy of NAb 2G8 evaluated with the indirect ELISA.

Figure 1 Binding efficacy of NAb 2G8 evaluated with the indirect ELISA.

NAb 2G8 exhibits high binding affinities with the CVA10 particles [median effective concentration (EC₅₀) values from 83.1 to 251.1 ng/ml]. The IC₅₀ and EC₅₀ values were calculated with nonlinear regression fitting curves. The optical density (OD) value was read at A₄₅₀/₆₂₀.

Zhu, R., Xu, L., Zheng, Q., Cui, Y., Li, S., He, M., ... & Chen, Z. (2018). Discovery and structural characterization of a therapeutic antibody against coxsackievirus A10. Science advances, 4(9), eaat7459.

Neut

Figure 2 Neutralization assay of CVA10 NAb 2G8.

Figure 2 Neutralization assay of CVA10 NAb 2G8.

The IC₅₀ value is 0.2 μg/ml.

Zhu, R., Xu, L., Zheng, Q., Cui, Y., Li, S., He, M., ... & Chen, Z. (2018). Discovery and structural characterization of a therapeutic antibody against coxsackievirus A10. Science advances, 4(9), eaat7459.

Neut

Figure 3 Neutralization assay of NAb 2G8 at pre-attachment (blue line) or post-attachment (red line).

Figure 3 Neutralization assay of NAb 2G8 at pre-attachment (blue line) or post-attachment (red line).

NAb 2G8 exhibits much higher neutralizing capacity at pre-attachment (IC₅₀ = 2.1 μg/ml) than at post-attachment (IC₅₀ = 22.7 μg/ml).

Zhu, R., Xu, L., Zheng, Q., Cui, Y., Li, S., He, M., ... & Chen, Z. (2018). Discovery and structural characterization of a therapeutic antibody against coxsackievirus A10. Science advances, 4(9), eaat7459.

FuncS

Figure 4 <em>In vivo</em> preventive and therapeutic efficacy of NAb 2G8.

Figure 4 In vivo preventive and therapeutic efficacy of NAb 2G8.

The infected mice were treated with NAb 2G8 12 hours before (blue line) or 24 hours after (red line) infection intraperitoneally with CVA10 and monitored daily after inoculation. Both experimental groups showed 100% survival rates, but 0% for two control groups.

Zhu, R., Xu, L., Zheng, Q., Cui, Y., Li, S., He, M., ... & Chen, Z. (2018). Discovery and structural characterization of a therapeutic antibody against coxsackievirus A10. Science advances, 4(9), eaat7459.


Specifications

  • Immunogen
  • CVA10 mature virions emulsified in an equal volume of Freund's adjuvant
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • CVA10
  • Species Reactivity
  • CVA10
  • Applications
  • ELISA, Neut, FuncS

Product Property

  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Storage
  • Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody 2G8 has been reported in applications of Enzyme-linked Immunosorbent Assay, Neutralization, Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
    The antibody can be diluted into 10⁻²-10⁶ ng/mL for Enzyme-linked Immunosorbent Assay and into 0.01-100 μg/mL for neutralization.

Target

  • Alternative Names
  • CVA10; Coxsackievirus A10

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Fab Antibody Products ScFv Antibody Products
CAT Product Name Application Type
PFBC-309 Recombinant Mouse Anti-CVA10 Antibody Fab Fragment (PFBC-309) ELISA, Neut Mouse Fab

Customer Reviews and Q&As

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  • User-Friendly Protocol
    The product comes with a user-friendly protocol that made it easy to learn how to use it effectively. This is a great help for someone new to this specific antibody.
  • Durable and Stable
    This antibody fragment proved to be durable and stable during long-term storage. I didn't observe any degradation, even after several months of use.
  • High Purity and Good Quality
    The antibody's purification quality is perfect. High purity enhances our assays' performance, producing reliable and reproducible results every time.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABC-309, RRID: AB_3111582)

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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