Recombinant Human Anti-DENV Antibody (m366) (CAT#: HPAB-AP058-YC)
Provided is a human anti-dengue virus antibody (an anti-DENV antibody) that binds to a DENV envelope protein and is cross-reactive with DENV serotype 1, DENV serotype 2, DENV serotype 3, and DENV serotype 4.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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SPR
Figure 1 Binding of m366 to DENV DIIIs from the four serotypes measured by Biacore.
The m366 was immobilized onto a CM5 chip, and the analytes consisted of serial dilution of DIII from DENV-1 (A), DENV-2 (B), DENV-3 (C), or DENV-4 (D). Binding kinetics was fitted using a 1:1 Langumir binding model by BIAevaluation 3.2 software.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
Specifications
- Host Species
- Human
- Derivation
- Human antibody library
- Specificity
- DENV
- Species Reactivity
- DENV
- Clone
- m366
- Applications
- SPR, ELISA, FC, Neut
Product Property
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles
Applications
- Application Notes
- The DENV antibody has been reported in applications of Surface Plasmon Resonance, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Neutralization.
For Surface Plasmon Resonance: Binding affinities of m360 scFv, m366 scFv, m360.6 scFv, and m366.6 scFv to the 4 DENV DIIIs were analyzed by surface plasmon resonance technology using a Biacore X100 instrument. The antibodies were covalently immobilized onto a sensor chip (CM5) using carbodiimide coupling chemistry. A control reference surface was prepared for nonspecific binding and refractive index changes. For analysis of the kinetics of interactions, varying concentrations of antigens were injected at flow rate of 30 μl/min using running buffer containing 10mM HEPES, 150 mM NaCl, 3 mM EDTA, and 0.05% Surfactant P-20 (pH 7.4). The association and dissociation phase data were fitted simultaneously to a 1:1 Langumir global model by using the nonlinear data analysis program BIAevaluation 3.2. All the experiments were done at 25°C.
Target
- Alternative Names
- Dengue virus
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibodies
Fab Antibodies
ScFv Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0154CQ | Recombinant Human Anti-DENV Antibody (MAb 1A5) | ELISA, Neut, WB, FuncS | Human IgG1 |
| HPAB-0128-YJ | Human Anti-DENV Recombinant Antibody (HPAB-0128-YJ) | ELISA, Neut, FuncS | Humanized IgG |
| HPAB-2089LY | Mouse Anti-DENV Recombinant Antibody (HPAB-2089LY) | ELISA, RIA, WB | Mouse IgG |
| HPAB-AP059-YC | Recombinant Human Anti-DENV Antibody (m366.6) | SPR, Neut, ELISA, Funcs, FC | |
| HPAB-AP060-YC | Recombinant Human Anti-DENV Antibody (m360) | SPR, ELISA, FC, Neut |
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For Research Use Only. Not For Clinical Use.
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