Recombinant Human Anti-DENV Antibody (m366) (CAT#: HPAB-AP058-YC)

• Host Species
• Human

• Derivation
• Human antibody library

• Specificity
• DENV

• Species Reactivity
• DENV

• Clone
• m366

• Applications
• SPR, ELISA, FC, Neut

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles

• Application Notes
• The DENV antibody has been reported in applications of Surface Plasmon Resonance, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Neutralization.
  For Surface Plasmon Resonance: Binding affinities of m360 scFv, m366 scFv, m360.6 scFv, and m366.6 scFv to the 4 DENV DIIIs were analyzed by surface plasmon resonance technology using a Biacore X100 instrument. The antibodies were covalently immobilized onto a sensor chip (CM5) using carbodiimide coupling chemistry. A control reference surface was prepared for nonspecific binding and refractive index changes. For analysis of the kinetics of interactions, varying concentrations of antigens were injected at flow rate of 30 μl/min using running buffer containing 10mM HEPES, 150 mM NaCl, 3 mM EDTA, and 0.05% Surfactant P-20 (pH 7.4). The association and dissociation phase data were fitted simultaneously to a 1:1 Langumir global model by using the nonlinear data analysis program BIAevaluation 3.2. All the experiments were done at 25°C.

• Alternative Names
• Dengue virus

• HPAB-AP058-YC-S(P)
• Recombinant Human Anti-DENV Antibody scFv Fragment (m366)

• HPAB-AP058-YC-F(E)
• Recombinant Human Anti-DENV Antibody Fab Fragment (m366)

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