Recombinant Human Anti-DENV Antibody (m360.6) (CAT#: HPAB-AP061-YC)
Provided is a human anti-dengue virus antibody (an anti-DENV antibody) that binds to a DENV envelope protein and is cross-reactive with DENV serotype 1, DENV serotype 2, DENV serotype 3, and DENV serotype 4.
Specific Inquiry
SPR
Figure 1 Binding of m360.6 to DENV DIIIs from the four serotypes measured by Biacore.
The m360.6 was immobilized onto a CM5 chip, and the analytes consisted of serial dilution of DIII from DENV-1 (A), DENV-2 (B), DENV-3 (C), or DENV-4 (D). Binding kinetics was fitted using a 1:1 Langumir binding model by BIAevaluation 3.2 software.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
Neut
Figure 2 Broad neutralization of the four DENV serotypes by m360.6 and m366.6.
Infectivity of DENV RVPs for all four serotypes. RVPs for DENV-1 (WestPac), DENV-2 (S16803), DENV-3 (CH53489) or DENV-4 (TVP360) were serially diluted in DMEM. BHK DC-SIGN cells were added and cells were cultured for 72 h. The cells were then lysed and examined for reporter expression. The independent neutralization experiments were performed in duplicate.
Hu, D., Zhu, Z., Li, S., Deng, Y., Wu, Y., Zhang, N., ... & Tian, X. (2019). A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III. PLoS pathogens, 15(6), e1007836.
Specifications
- Host Species
- Human
- Derivation
- Human antibody library
- Specificity
- DENV
- Species Reactivity
- DENV
- Clone
- m360.6
- Applications
- SPR, Neut, ELISA, FC
Product Property
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles
Applications
- Application Notes
- The DENV antibody has been reported in applications of Surface Plasmon Resonance, Neutralization, Enzyme-linked Immunosorbent Assay, Flow Cytometry.
For Surface Plasmon Resonance: Binding affinities of m360 scFv, m366 scFv, m360.6 scFv, and m366.6 scFv to the 4 DENV DIIIs were analyzed by surface plasmon resonance technology using a Biacore X100 instrument. The antibodies were covalently immobilized onto a sensor chip (CM5) using carbodiimide coupling chemistry. A control reference surface was prepared for nonspecific binding and refractive index changes. For analysis of the kinetics of interactions, varying concentrations of antigens were injected at flow rate of 30 μl/min using running buffer containing 10mM HEPES, 150 mM NaCl, 3 mM EDTA, and 0.05% Surfactant P-20 (pH 7.4). The association and dissociation phase data were fitted simultaneously to a 1:1 Langumir global model by using the nonlinear data analysis program BIAevaluation 3.2. All the experiments were done at 25°C.
Target
- Alternative Names
- Dengue virus
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-416CQ | Recombinant Mouse Anti-DENV Antibody (5161) | ELISA | Mouse antibody |
| MRO-417CQ | Recombinant Mouse Anti-DENV Antibody (MRO-417CQ) | ELISA | Mouse antibody |
| MRO-418CQ | Recombinant Mouse Anti-DENV Antibody (MRO-418CQ) | WB, IF, IHC-P | Mouse antibody |
| MRO-419CQ | Recombinant Mouse Anti-DENV Antibody (MRO-419CQ) | Dot, ELISA, IF | Mouse antibody |
| MRO-420CQ | Recombinant Mouse Anti-DENV Antibody (D1-11(3)) | Dot, ELISA, IF, WB | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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