Rabbit Anti-DGCR8 Recombinant Antibody (clone 2H2) (CAT#: ZG-0635J)

Name: Rabbit Anti-DGCR8 Recombinant Antibody (clone 2H2)
SKU: ZG-0635J
Rating: 5 (1 reviews)

This product is a rabbit antibody that recognizes DGCR8.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Rabbit Anti-DGCR8 Antibody (ZG-0635J) in WB

Figure 1 Rabbit Anti-DGCR8 Antibody (ZG-0635J) in WB

Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: DGCR8 antibody at 2.65μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87, 33, 83 KDa
Observed band size: 100 KDa

Subcellular Location

Figure 1 IF staining of human cell line REH
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76916/1779_F9_2_selected.jpg

Normal Tissue

Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79351/170060_A_6_6_selected.jpg

Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79351/170060_B_9_5.jpg

Figure 4 Colon
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79351/170060_A_9_3.jpg

Figure 5 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79351/170060_A_8_5.jpg

Figure 6 Testis
(Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79351/170060_A_6_6.jpg

Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79351/170060_A_9_8.jpg

RNA Expression

Figure 8 RNA cell line category: Cell line enhanced (K-562)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128191-DGCR8

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthesized peptide derived from human DGCR8

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human DGCR8

Species Reactivity
Human

Clone
2H2

Applications
ELISA, WB

Conjugate
Uconjugated

Product Property

Purification
Affinity purified

Format
Liquid

Concentration
See the COA

Buffer
PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preservative
0.02% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot.
WB:1:500-1:5000

Target

Alternative Names
Gy1; pasha; DGCRK6; C22orf12; microprocessor complex subunit DGCR8; DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8

Gene ID
54487

UniProt ID
Q8WYQ5

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "DGCR8"

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IgG Antibody Products

CAT	Product Name	Application	Type
MRO-0475-CN	Rabbit Anti-DGCR8 Recombinant Antibody (clone CBACN-195)	WB, IF, IP	Rabbit IgG
VS3-WK1080	Rabbit Anti-DGCR8 Recombinant Antibody (VS3-WK1080)	WB, ICC, IF, IP	Rabbit IgG
VS3-FY452	Recombinant Rabbit Anti-DGCR8 Antibody (clone R05-5A6)	WB, ICC, IF, IP	Rabbit IgG

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