Rabbit Anti-DGKA Recombinant Antibody (clone R06-6H4) (CAT#: VS3-FY1996)
The antibody is verification in western blot analysis of DGKA in Hela, A549 lysates. The recombinant monoclonal antibody is specifically created to bind human DGKA. It is suitable for use in a variety of assays, including WB, IHC-P, IP. The R06-6H4 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunoprecipitation (dilution 1/20).
Specific Inquiry
WB
Figure 1 Anti-DGKA Antibody (VS3-FY1996) in WB.
The DGKA expression in Hela, A549 lysates were detected using the anti-DGKA by western blot assay.
Specifications
- Immunogen
- Recombinant human DGKA protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DGKA
- Species Reactivity
- Human
- Clone
- R06-6H4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 83 kDa, while the observed molecular weight stands at 83 kDa.
- Related Disease
- Lymphoproliferative Syndrome, X-Linked, 1 and Bardet-Biedl Syndrome 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Diacylglycerol Kinase Alpha; Diacylglycerol Kinase, Alpha 80kDa; 80 KDa Diacylglycerol Kinase; Diglyceride Kinase Alpha; DAG Kinase Alpha; EC 2.7.1.107
- Gene ID
- 1606
- UniProt ID
- P23743
- Sequence Similarities
- Belongs to the eukaryotic diacylglycerol kinase family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys18, Lys239, Lys249, Lys263, Lys353, Lys411, Lys484, Lys493, and Lys634 (NX_P23743 [NX_P23743-1])
Modification sites at PhosphoSitePlus (P23743)
Glycosylation from GlyGen (P23743) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011536297.1; XP_016874389.1; XP_016874390.1; XP_016874391.1; XP_016874396.1
- Function
- Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes.
Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "DGKA"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3309z | Mouse Anti-DGKA Recombinant Antibody (clone 8H3) | ELISA, IHC, FuncS | Mouse IgG2b, κ |
| ZG-1047F | Mouse Anti-DGKA Recombinant Antibody (clone M5a) | IP, WB | Mouse IgG2a |
| ZG-1048F | Mouse Anti-DGKA Recombinant Antibody (clone M6) | IP, WB | Mouse IgG1 |
| ZG-1049F | Mouse Anti-DGKA Recombinant Antibody (clone M8) | IP, WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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