Rabbit Anti-DGKA Recombinant Antibody (clone R06-6H4)
CAT#: VS3-FY1996
The antibody is verification in western blot analysis of DGKA in Hela, A549 lysates. The recombinant monoclonal antibody is specifically created to bind human DGKA. It is suitable for use in a variety of assays, including WB, IHC-P, IP. The R06-6H4 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunoprecipitation (dilution 1/20).
Tested Data
Gene Expression
WB
Figure 1 Anti-DGKA Antibody (VS3-FY1996) in WB.
The DGKA expression in Hela, A549 lysates were detected using the anti-DGKA by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45462/1541_D1_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41645/88612_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41645/88612_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41645/88612_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41645/88612_A_6_6.jpg
Normal Tissue
Figure 6 Tonsil
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41645/88612_A_6_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HBEC3-KT, hTERT-HME1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065357-DGKA
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Specifications
- Immunogen
- Recombinant human DGKA protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DGKA
- Species Reactivity
- Human
- Clone
- R06-6H4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 83 kDa, while the observed molecular weight stands at 83 kDa.
- Related Disease
- Lymphoproliferative Syndrome, X-Linked, 1 and Bardet-Biedl Syndrome 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Diacylglycerol Kinase Alpha; Diacylglycerol Kinase, Alpha 80kDa; 80 KDa Diacylglycerol Kinase; Diglyceride Kinase Alpha; DAG Kinase Alpha; EC 2.7.1.107
- Gene ID
- 1606
- UniProt ID
- P23743
- Sequence Similarities
- Belongs to the eukaryotic diacylglycerol kinase family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys18, Lys239, Lys249, Lys263, Lys353, Lys411, Lys484, Lys493, and Lys634 (NX_P23743 [NX_P23743-1])
Modification sites at PhosphoSitePlus (P23743)
Glycosylation from GlyGen (P23743) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011536297.1; XP_016874389.1; XP_016874390.1; XP_016874391.1; XP_016874396.1
- Function
- Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes.
Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "DGKA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3309z | Mouse Anti-DGKA Recombinant Antibody (clone 8H3) | ELISA, IHC, FuncS | Mouse IgG2b, κ |
| ZG-1047F | Mouse Anti-DGKA Recombinant Antibody (clone M5a) | IP, WB | Mouse IgG2a |
| ZG-1048F | Mouse Anti-DGKA Recombinant Antibody (clone M6) | IP, WB | Mouse IgG1 |
| ZG-1049F | Mouse Anti-DGKA Recombinant Antibody (clone M8) | IP, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY77 | AbPlus™ Anti-DGKA Magnetic Beads (M5a) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1981 | Anti-DGKA Immunohistochemistry Kit | IHC |
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