Anti-DKK2 Antibody Prodrug, Protease Activated (VS-1025-YC143)
CAT#: VS-1025-YC143
The Anti-DKK2 Antibody Prodrug, Protease Activated (VS-1025-YC143) is an antibody prodrug comprised of a recombinant human antibody targeting human DKK2, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to DKK2 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to DKK2 effectively. DKK2 is a secreted antagonist that acts as a negative regulator of the canonical Wnt signaling pathway, which is frequently dysregulated in cancer. DKK2's role in tumorigenesis is context-dependent; while it typically inhibits Wnt signaling and can function as a tumor suppressor, it can also promote cancer growth in certain settings by activating the non-canonical Wnt pathway. It is often overexpressed in the tumor microenvironment of cancers like multiple myeloma, where its activity contributes to bone destruction.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52611/1697_G5_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows moderate cytoplasmic positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9727/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9727/33036_B_7_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9727/33036_A_4_6.jpg
Normal Tissue
Figure 5 Cervix
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9727/33036_B_8_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (SH-SY5Y)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000155011-DKK2
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human DKK2
- Target
- DKK2
- Species Reactivity
- Human
- Isotype
- IgG1, kappa
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6).
- Alternative Names
- DKK-2
- Gene ID
- 27123
- UniProt ID
- Q9UBU2
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-1025-YC143. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "DKK2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0057CQ | Human Anti-DKK2 Recombinant Antibody (HPAB-0057CQ) | ELISA, FC, Neut | Humanized IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0057CQ-S(P) | Human Anti-DKK2 Recombinant Antibody; scFv Fragment (HPAB-0057CQ-S(P)) | ELISA, FC, Neut | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0057CQ-F(E) | Human Anti-DKK2 Recombinant Antibody; Fab Fragment (HPAB-0057CQ-F(E)) | ELISA, FC, Neut | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1189 | Mouse Anti-DKK2 Recombinant Antibody (VS3-WK1189) | WB | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC90 | Recombinant Anti-DKK2 Eliminating Antibody, pH-Sensitive (VS-0625-YC90) | Antigen-Sweeping In Vivo. | Human IgG1 kappa |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, Cyt, Stim, PP, Agonist
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
