Mouse Anti-DNAJB1 Recombinant Antibody (clone 3G2)
CAT#: VS3-WK1620
This antibody is a mouse recombinant antibody against human DNAJB1, which is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. The clone 3G2 can be used in WB analysis.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63247/if_selected.jpg
Normal Tissue
Figure 2 Adrenal gland
High expression in adrenal gland
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63247/143115_B_5_5_val_selected.jpg
Normal Tissue
Figure 3 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63247/143115_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63247/143115_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63247/143115_B_9_5.jpg
Normal Tissue
Figure 6 Esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63247/143115_B_4_1.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63247/143115_A_9_4.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132002-DNAJB1
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Specifications
- Immunogen
- Recombinant human HSP40 protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human DNAJB1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 3G2
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Lyophilized powder
- Buffer
- Lyophilized from 0.01M TBS (pH 7.4) with 1% BSA and 50% Glycerol
- Preservative
- 0.03% Proclin300
- Storage
- Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted the antibody is stable for at least two weeks at 2-4°C.
- Shipping
- Ice packs
Target
- Alternative Names
- Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
- Gene ID
- 3337
- UniProt ID
- P25685
- Long Name
- DnaJ heat shock protein family (Hsp40) member B1
- Cellular Localization
- Cytoplasm, Nucleus
- Protein Refseq
- NP_001287843.1
- Function
- Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "DNAJB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3307z | Mouse Anti-DNAJB1 Recombinant Antibody (clone 18B2) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-0878CT | Recombinant Mouse anti-Human DNAJB1 Monoclonal antibody (2I9) | FC, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1013 | Hi-Affi™ Rabbit Anti-DNAJB1 Recombinant Antibody (clone DS1013AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY262 | Rabbit Anti-NHP DNAJB1 Recombinant Antibody (VS-1024-XY262) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY680 | Anti-DNAJB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2032 | Anti-Mouse DNAJB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2031 | Anti-Human DNAJB1 Immunohistochemistry Kit | IHC |
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