Rabbit Anti-DOHH Recombinant Antibody (clone R04-4I8) (CAT#: VS3-FY1702)
This anti-DOHH antibody detected positive in western blot analysis of DOHH in K562, Hela lysates. A remarkable rabbit recombinant monoclonal antibody that is created to detect human DOHH. It is suitable for use in a variety of assays, including WB, IP.
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WB
Figure 1 Anti-DOHH Antibody (VS3-FY1702) in WB.
The DOHH expression in K562, Hela lysates were detected using the anti-DOHH by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human DOHH
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DOHH
- Species Reactivity
- Human
- Clone
- R04-4I8
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 33 kDa, while the observed molecular weight stands at 33 kDa.
- Related Disease
- Neurodevelopmental Disorder With Microcephaly, Cerebral Atrophy, And Visual Impairment and Non-Specific Syndromic Intellectual Disability
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Deoxyhypusine Hydroxylase; HEAT-Like (PBS Lyase) Repeat Containing 1; Deoxyhypusine Hydroxylase/Monooxygenase; HEAT-Like Repeat-Containing Protein 1; Deoxyhypusine Monooxygenase; Deoxyhypusine Dioxygenase; EC 1.14.99.29; HLRC1; HDOHH
- Gene ID
- 83475
- UniProt ID
- Q9BU89
- Sequence Similarities
- Belongs to the deoxyhypusine hydroxylase family.
- Cellular Localization
- Cytosol
- Post Translation Modifications
- Acetylation
- Protein Refseq
- XP_011526643.1; XP_054178268.1; NP_001138637.1; NP_112594.1
- Function
- Catalyzes the hydroxylation of the N6-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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