Rabbit Anti-DOHH Recombinant Antibody (clone R04-4I8) (CAT#: VS3-FY1702)
This anti-DOHH antibody detected positive in western blot analysis of DOHH in K562, Hela lysates. A remarkable rabbit recombinant monoclonal antibody that is created to detect human DOHH. It is suitable for use in a variety of assays, including WB, IP.
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WB
Figure 1 Anti-DOHH Antibody (VS3-FY1702) in WB.
The DOHH expression in K562, Hela lysates were detected using the anti-DOHH by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human DOHH
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DOHH
- Species Reactivity
- Human
- Clone
- R04-4I8
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 33 kDa, while the observed molecular weight stands at 33 kDa.
- Related Disease
- Neurodevelopmental Disorder With Microcephaly, Cerebral Atrophy, And Visual Impairment and Non-Specific Syndromic Intellectual Disability
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Deoxyhypusine Hydroxylase; HEAT-Like (PBS Lyase) Repeat Containing 1; Deoxyhypusine Hydroxylase/Monooxygenase; HEAT-Like Repeat-Containing Protein 1; Deoxyhypusine Monooxygenase; Deoxyhypusine Dioxygenase; EC 1.14.99.29; HLRC1; HDOHH
- Gene ID
- 83475
- UniProt ID
- Q9BU89
- Sequence Similarities
- Belongs to the deoxyhypusine hydroxylase family.
- Cellular Localization
- Cytosol
- Post Translation Modifications
- Acetylation
- Protein Refseq
- XP_011526643.1; XP_054178268.1; NP_001138637.1; NP_112594.1
- Function
- Catalyzes the hydroxylation of the N6-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
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