Rabbit Anti-DOHH Recombinant Antibody (clone R04-4I8)

CAT#: VS3-FY1702

This anti-DOHH antibody detected positive in western blot analysis of DOHH in K562, Hela lysates. A remarkable rabbit recombinant monoclonal antibody that is created to detect human DOHH. It is suitable for use in a variety of assays, including WB, IP.

Tested Data
Figure 1 Anti-DOHH Antibody (VS3-FY1702) in WB.

Specifications

  • Immunogen
  • Synthetic peptide of human DOHH
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human DOHH
  • Species Reactivity
  • Human
  • Clone
  • R04-4I8
  • Applications
  • Western Blot, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 33 kDa, while the observed molecular weight stands at 33 kDa.
  • Related Disease
  • Neurodevelopmental Disorder With Microcephaly, Cerebral Atrophy, And Visual Impairment and Non-Specific Syndromic Intellectual Disability

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Deoxyhypusine Hydroxylase; HEAT-Like (PBS Lyase) Repeat Containing 1; Deoxyhypusine Hydroxylase/Monooxygenase; HEAT-Like Repeat-Containing Protein 1; Deoxyhypusine Monooxygenase; Deoxyhypusine Dioxygenase; EC 1.14.99.29; HLRC1; HDOHH
  • Sequence Similarities
  • Belongs to the deoxyhypusine hydroxylase family.
  • Cellular Localization
  • Cytosol
  • Post Translation Modifications
  • Acetylation
  • Function
  • Catalyzes the hydroxylation of the N6-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

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