Rabbit Anti-DPYSL4 Recombinant Antibody (clone R01-6G1) (CAT#: VS3-FY1688)
This rat DPYSL4 recombinant monoclonal antibody is purified by affinity chromatography. This product is a extraordinary rabbit recombinant monoclonal antibody that detects human and rat DPYSL4 of calculated molecular weight at 316 kDa. The R01-6G1 is recommended for dilution with western blot (dilution 1/500-1/1000).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/752_D7_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/752_D7_2_selected.jpg
Figure 2 IHC staining of human hippocampus
(Immunohistochemical staining of human hippocampus shows strong cytoplasmic and nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/ihc_selected.jpg
(Immunohistochemical staining of human hippocampus shows strong cytoplasmic and nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/ihc_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/114493_B_9_5.jpg
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/114493_B_9_5.jpg
Figure 4 Cerebellum
(Cells in granular layer Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Cells in molecular layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Purkinje cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/114493_B_8_8.jpg
(Cells in granular layer Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Cells in molecular layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Purkinje cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/114493_B_8_8.jpg
Figure 5 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/114493_A_8_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/114493_A_8_3.jpg
Figure 6 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/114493_A_8_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/114493_A_8_4.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/114493_A_9_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/114493_A_9_5.jpg
Figure 8 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/114493_A_6_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/114493_A_6_6.jpg
Figure 9 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/114493_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49066/114493_A_8_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (AF22, AN3-CA, SH-SY5Y)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000151640-DPYSL4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000151640-DPYSL4
Specifications
- Immunogen
- Synthetic peptide of human CRMP3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat DPYSL4
- Species Reactivity
- Human, Rat
- Clone
- R01-6G1
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 62 kDa, while the observed molecular weight stands at 62 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Dihydropyrimidinase Like 4; Collapsin Response Mediator Protein 3; UNC33-Like Phosphoprotein 4; CRMP-3; ULIP-4; CRMP3
- Gene ID
- 10570
- UniProt ID
- O14531
- Sequence Similarities
- Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- XP_054220473.1; XP_054220474.1; XP_054220475.1; NP_006417.2
- Function
- Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1045 | Hi-Affi™ Rabbit Anti-DPYSL4 Recombinant Antibody (clone DS1045AB) | WB | Rabbit IgG |
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