How should my assay handle non-specific binding?

Check the concentration of the antibodies, employ the appropriate controls, and adhere to the correct blocking and washing protocols. Reduced primary antibody concentration may also be advantageous.

Can very small levels of proteins be detected by this antibody?

Yes, but you might need to use high-sensitivity detection techniques, adjust the assay parameters, or increase the sample amount in order to improve the signal.

How can I select the right lysis buffer for my specimens?

The type of lysis buffer you employ depends on the location and characteristics of your target protein. For example, RIPA buffer is effective when working with both nuclear and cytoplasmic proteins.

Human Anti-E. coli BRIL Recombinant Antibody (clone BAG2); Fab Fragment

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Popular Searches: Recombinant Antibody MHC Tetramer T cell Receptor Scaffold Protein

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

See other products for "E. coli BRIL"

CAT	Product Name	Application	Type
PABS-0175	Human Anti-E. coli BRIL Recombinant Antibody (clone BAK5)	SPR, ELISA	Human IgG
PABJ-0171	Human Anti-E. coli BRIL Recombinant Antibody (clone BAG2)	ELISA	Human IgG

CAT

Product Name

Application

Type

PABS-0175

Human Anti-E. coli BRIL Recombinant Antibody (clone BAK5)

SPR, ELISA

Human IgG

PABJ-0171

Human Anti-E. coli BRIL Recombinant Antibody (clone BAG2)

ELISA

Human IgG

Customer Reviews and Q&As

CAT	Product Name	Application	Type
PABJ-0171-S(P)	Human Anti-E. coli BRIL Recombinant Antibody (clone BAG2); scFv Fragment	ELISA	Human scFv

Customer Review Q&As

Recurring Results

We conducted multiple experiments with this antibody, and the outcomes were reliable. Extremely happy with the constancy.

Excellent Signal-to-Noise

The signal-to-noise ratio for our fluorescence microscopy investigations was good. really efficient and dependable.

Entire Processes

The guidelines that were supplied were easy to follow and comprehend. They made it very simple to integrate the antibody into our processes.

Q 1: How should my assay handle non-specific binding?
A: Check the concentration of the antibodies, employ the appropriate controls, and adhere to the correct blocking and washing protocols. Reduced primary antibody concentration may also be advantageous.
Q 2: Can very small levels of proteins be detected by this antibody?
A: Yes, but you might need to use high-sensitivity detection techniques, adjust the assay parameters, or increase the sample amount in order to improve the signal.
Q 3: How can I select the right lysis buffer for my specimens?
A: The type of lysis buffer you employ depends on the location and characteristics of your target protein. For example, RIPA buffer is effective when working with both nuclear and cytoplasmic proteins.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABJ-0171-F(E), RRID: AB_3111614)

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