Human Anti-E. coli MBP Recombinant Antibody (Clone sAB11M) (CAT#: PABS-0121)
This recombinant monoclonal antibody to E. coli MBP is a human monoclonal antibody that can be used for applications: ELISA.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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SDS-PAGE
Figure 1 Human Anti-E. coli MBP Recombinant Antibody (Clone sAB11M) (PABS-0121) in SDS-PAGE
SDS-PAGE analysis of PABS-0121 in β-mercaptoethanol-reduced (Lane 2) and non-reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 180-275 kDa.
SEC-HPLC
Figure 2 Human Anti-E. coli MBP Recombinant Antibody (Clone sAB11M) (PABS-0121) in SEC-HPLC
The purity of PABS-0121 was greater than 98% as determined by SEC-HPLC.
WB
Figure 3 Human Anti-E. coli MBP Recombinant Antibody (Clone sAB11M) (PABS-0121) in WB
Western blot analysis of PABS-0121 was performed by loading E. coli MBP protein, His tag.
PABS-0121 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L).
Lane 1: Reducing Antigen (0.5 μg)
Lane 2: Reducing Antigen (1 μg)
ELISA
Figure 4 Human Anti-E. coli MBP Recombinant Antibody (Clone sAB11M) (PABS-0121) in ELISA
ELISA analysis of PABS-0121 was performed by coating with E. coli MBP protein (His tag). Then blocked with BSA and incubated with PABS-0121. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- E. coli MBP
- Species Reactivity
- E. coli
- Clone
- sAB11M
- Applications
- ELISA, FuncS
- Related Disease
- E. coli infections
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- maltose-binding protein; MMBP; Maltodextrin-binding protein; malE
- Gene ID
- 948538
- UniProt ID
- P0AEX9
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-833-sdAb | Recombinant Anti-E. coli MBP VHH Single Domain Antibody | ICC, FC, WB, FUNC | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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