Human Anti-E. coli MBP Recombinant Antibody (Clone sAB11M) (CAT#: PABS-0121)
This recombinant monoclonal antibody to E. coli MBP is a human monoclonal antibody that can be used for applications: ELISA.
Specific Inquiry
ELISA
Figure 1 Characterization of conformation-specific sABs
Phage ELISA shows that sABs generated against the maltose-bound, closed form of MBP do not bind or bind with very reduced affinity to the open form of MBP.
Mukherjee, S., Griffin, D. H., Horn, J. R., Rizk, S. S., Nocula-Lugowska, M., Malmqvist, M., ... & Kossiakoff, A. A. (2018). Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins. Journal of Biological Chemistry, 293(8), 2815-2828.
ELISA
Figure 2 single-point ELISA shows that the binding of sAB-7O and sAB-11M are exclusive.
Neither of them binds in the presence of an excess of the other. the sAB of interest was His-tagged, and the ELISA signal (A450) was monitored using anti-His antibody.
Mukherjee, S., Griffin, D. H., Horn, J. R., Rizk, S. S., Nocula-Lugowska, M., Malmqvist, M., ... & Kossiakoff, A. A. (2018). Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins. Journal of Biological Chemistry, 293(8), 2815-2828.
Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- E. coli MBP
- Species Reactivity
- E. coli
- Clone
- sAB11M
- Applications
- ELISA, FuncS
- Related Disease
- E. coli infections
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- maltose-binding protein; MMBP; Maltodextrin-binding protein; malE
- Gene ID
- 948538
- UniProt ID
- P0AEX9
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-833-sdAb | Recombinant Anti-E. coli MBP VHH Single Domain Antibody | ICC, FC, WB, FUNC | Llama VHH |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1079 | Hi-Affi™ Recombinant Rabbit Anti-E.coli MBP Monoclonal Antibody (DS1079AB) | WB | IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBS-0121 | Human Anti-E. coli MBP Recombinant Antibody (Clone sAB11M); Fab Fragment | ELISA, FuncS | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBS-0121 | Human Anti-E. coli MBP Recombinant Antibody (Clone sAB11M); scFv Fragment | ELISA, FuncS | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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