Human Anti-E. coli MBP Recombinant Antibody (Clone sAB11M) (CAT#: PABS-0121)
This recombinant monoclonal antibody to E. coli MBP is a human monoclonal antibody that can be used for applications: ELISA.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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SDS-PAGE
Figure 1 Human Anti-E. coli MBP Recombinant Antibody (Clone sAB11M) (PABS-0121) in SDS-PAGE
SDS-PAGE analysis of PABS-0121 in β-mercaptoethanol-reduced (Lane 2) and non-reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 180-275 kDa.
SEC-HPLC
Figure 2 Human Anti-E. coli MBP Recombinant Antibody (Clone sAB11M) (PABS-0121) in SEC-HPLC
The purity of PABS-0121 was greater than 98% as determined by SEC-HPLC.
WB
Figure 3 Human Anti-E. coli MBP Recombinant Antibody (Clone sAB11M) (PABS-0121) in WB
Western blot analysis of PABS-0121 was performed by loading E. coli MBP protein, His tag.
PABS-0121 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L).
Lane 1: Reducing Antigen (0.5 μg)
Lane 2: Reducing Antigen (1 μg)
ELISA
Figure 4 Human Anti-E. coli MBP Recombinant Antibody (Clone sAB11M) (PABS-0121) in ELISA
ELISA analysis of PABS-0121 was performed by coating with E. coli MBP protein (His tag). Then blocked with BSA and incubated with PABS-0121. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- E. coli MBP
- Species Reactivity
- E. coli
- Clone
- sAB11M
- Applications
- ELISA, FuncS
- Related Disease
- E. coli infections
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- maltose-binding protein; MMBP; Maltodextrin-binding protein; malE
- Gene ID
- 948538
- UniProt ID
- P0AEX9
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-833-sdAb | Recombinant Anti-E. coli MBP VHH Single Domain Antibody | ICC, FC, WB, FUNC | Llama VHH |
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