Mouse Anti-E-Tag Recombinant Antibody (clone 11H12B3) (CAT#: ZG-0287U)
Mouse anti-E-Tag antibody (clone 11H12B3) displayed high specificity against the E-Tag.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Mouse Anti-E-Tag Antibody (ZG-0287U) in WB
Positive WB detected in: 1-3 lanes: E-tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag All lanes: E-Tag antibody at 1: 1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 30, 68, 55, 67-157kDa
Observed band size: 30, 68, 55, 67-157kDa
Figure 2 Mouse Anti-E-Tag Antibody (ZG-0287U) in IP
Immunoprecipitating E-Tag in 293F transfected whole cell lysate Lane 1: Mouse control IgG (1µg) in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0287U (5µg) + 293F transfected whole cell lysate (500µg)
Lane 3: 293F transfected whole cell lysate (20µg)
Figure 3 Mouse Anti-E-Tag Antibody (ZG-0287U) in ELISA
ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag) Antigen at dilution of 1µg/ml
E-Tag antibody at (0-0.15µg)
Goat polyclonal to Mouse IgG at 1/50000 dilution
Specifications
- Immunogen
- GAPVPYPDPLEPRC synthetic peptide conjugate to KLH
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- E-Tag
- Species Reactivity
- All
- Clone
- 11H12B3
- Applications
- ELISA, WB, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation. Specific conditions should be optimized by the end user.
ELISA:1:4000-1:8000, WB:1:1000-1:320000, IP:1µg-5µg
Target
- Alternative Names
- E-Tag
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Customer Reviews and Q&As
Time-Saving
Highly Recommended
Stable Over Time
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# ZG-0287U, RRID: AB_3112066)
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For Research Use Only. Not For Clinical Use.
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