Mouse Anti-EBFP Recombinant Antibody (clone 3A6)
CAT#: ZG-411C
This product is a mouse antibody that recognizes EBFP. It can be used for ELISA, IB and IP.
Tested Data
ELISA
Figure 1 Mouse Anti-EBFP Antibody (ZG-411C) in ELISA
Antibody was tested on various recombinant protein substrates i.e. BSA, 98% (Sigma), HIS6-hRrp4 (pET15b), GST-hRpp25 (pGEX-2T), GST-hRrp4 (pGEX-2T), HIS10-hRpp25 (pET16b), and a biotin containing peptide (22 mer).
Immunoblot
Figure 2 Mouse Anti-EBFP Antibody (ZG-411C) in Immunoblot
Blot contains total cell extract containing recombinant EGFP (predicted band size 27 kDa). Incubated with antibody fraction (0.5 mg/ml) 1000X diluted in PBS containing 0,05% tween-20 and 5% non fat dry milk.
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Specifications
- Immunogen
- Recombinant EBFP (NCBI accession number AX_766758 REGION:1-717, expression vector pGEX-1N), expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- In immunoblot the antibody cross reacts with GFP and YFP.
- Clone
- 3A6
- Applications
- ELISA, IB, IP
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as deterimined by SDS-PAGE
- Format
- Lyophilized
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Target
- Alternative Names
- Enhanced blue fluorescent protein
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "EBFP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0005C | Mouse Anti-EBFP Antibody (clone Mix) | WB | Mouse IgG |
| ZG-412C | Mouse Anti-EBFP Recombinant Antibody (clone 7F10) | ELISA | Mouse IgG1 |
| VS3-WK1341 | Mouse Anti-EBFP Recombinant Antibody (VS3-WK1341) | WB | Mouse IgG |
| VS3-WK1425 | Mouse Anti-EBFP Antibody (clone Mix-mA) | WB, IHC, IP | Mouse IgG |
| VS4-WK151 | Mouse Anti-EBFP Recombinant Antibody (VS4-WK151) | ELISA, WB | Mouse IgG |
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