Mouse Anti-EBFP Recombinant Antibody (clone 3A6) (CAT#: ZG-411C)

This product is a mouse antibody that recognizes EBFP. It can be used for ELISA, IB and IP.


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ELISA

Figure 1 Mouse Anti-EBFP Antibody (ZG-411C) in ELISA

Figure 1 Mouse Anti-EBFP Antibody (ZG-411C) in ELISA

Antibody was tested on various recombinant protein substrates i.e. BSA, 98% (Sigma), HIS6-hRrp4 (pET15b), GST-hRpp25 (pGEX-2T), GST-hRrp4 (pGEX-2T), HIS10-hRpp25 (pET16b), and a biotin containing peptide (22 mer).

Immunoblot

Figure 2 Mouse Anti-EBFP Antibody (ZG-411C) in Immunoblot

Figure 2 Mouse Anti-EBFP Antibody (ZG-411C) in Immunoblot

Blot contains total cell extract containing recombinant EGFP (predicted band size 27 kDa). Incubated with antibody fraction (0.5 mg/ml) 1000X diluted in PBS containing 0,05% tween-20 and 5% non fat dry milk.


Specifications

  • Immunogen
  • Recombinant EBFP (NCBI accession number AX_766758 REGION:1-717, expression vector pGEX-1N), expressed in E. coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • In immunoblot the antibody cross reacts with GFP and YFP.
  • Clone
  • 3A6
  • Applications
  • ELISA, IB, IP

Product Property

  • Purification
  • Protein A affinity purified
  • Purity
  • >95% as deterimined by SDS-PAGE
  • Format
  • Lyophilized
  • Storage
  • Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.

Target

  • Alternative Names
  • Enhanced blue fluorescent protein

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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