Mouse Anti-EBNA-1 Recombinant Antibody (clone 16D2)
CAT#: FAMAB-0524WJ
This product is a mouse monoclonal antibody that is specific for EBV EBNA1. This antibody can be used in a variety of applications, such as ELISA, WB, IF.
Published Data
ELISA
Figure 1 IgG antibody to EBNA‐1 cross‐reacts with dsDNA.
IgG MAb, 3D4 binds strongly to EBNA-1 (A) and cross-reacts with dsDNA (B) as determined by ELISA. ELISAs are representative of three experiments.
Yadav, P., Carr, M. T., Yu, R., Mumbey‐Wafula, A., & Spatz, L. A. (2016). Mapping an epitope in EBNA‐1 that is recognized by monoclonal antibodies to EBNA‐1 that cross‐react with dsDNA. Immunity, inflammation and disease, 4(3), 362-375.
ELISA
Figure 2 16D2 was tested by ELISA for binding to EBNA‐1.
Yadav, P., Carr, M. T., Yu, R., Mumbey‐Wafula, A., & Spatz, L. A. (2016). Mapping an epitope in EBNA‐1 that is recognized by monoclonal antibodies to EBNA‐1 that cross‐react with dsDNA. Immunity, inflammation and disease, 4(3), 362-375.
ELISA
Figure 3 16D2 was tested by ELISA for binding to dsDNA.
Yadav, P., Carr, M. T., Yu, R., Mumbey‐Wafula, A., & Spatz, L. A. (2016). Mapping an epitope in EBNA‐1 that is recognized by monoclonal antibodies to EBNA‐1 that cross‐react with dsDNA. Immunity, inflammation and disease, 4(3), 362-375.
IF
Figure 4 Binding to dsDNA was confirmed by immunostaining of the kinetoplast of Crithidia luciliae (C). Immunostaining of an isotype control MAb is shown in D (D).
Yadav, P., Carr, M. T., Yu, R., Mumbey‐Wafula, A., & Spatz, L. A. (2016). Mapping an epitope in EBNA‐1 that is recognized by monoclonal antibodies to EBNA‐1 that cross‐react with dsDNA. Immunity, inflammation and disease, 4(3), 362-375.
ELISA
Figure 5 16D2 was tested by ELISA for reactivity with a panel of antigens; Sm, LPS, BSA, and proteinase 3 (PR3).
Yadav, P., Carr, M. T., Yu, R., Mumbey‐Wafula, A., & Spatz, L. A. (2016). Mapping an epitope in EBNA‐1 that is recognized by monoclonal antibodies to EBNA‐1 that cross‐react with dsDNA. Immunity, inflammation and disease, 4(3), 362-375.
ELISA
Figure 6 16D2 binds to an epitope in the carboxyl region of EBNA‐1.
(A) Open reading frame map of EBNA-1 showing the amino (LS8) and carboxyl (LS9) region. (B) 16D2 was tested by ELISA for binding to LS8 and LS9 and shown to bind LS9 only. (C) Schematic of three truncated recombinant fragments of LS9 expressed in and purified from E. coli. (D) 16D2 was tested by ELISA for binding to the truncated fragments, aa 452-641, aa 459-619, and aa 459-607 and compared to the binding to LS9 (aa 410-641).
Yadav, P., Carr, M. T., Yu, R., Mumbey‐Wafula, A., & Spatz, L. A. (2016). Mapping an epitope in EBNA‐1 that is recognized by monoclonal antibodies to EBNA‐1 that cross‐react with dsDNA. Immunity, inflammation and disease, 4(3), 362-375.
ELISA
Figure 7 Comparison of the binding of 3D4 and 16D2 to EBNA‐1.
The binding of 16D2 and 3D4 to EBNA-1were compared by ELISA. Goat anti-mouse kappa-AP was used as secondary antibody to detect the binding of 16D2 (an IgM MAb) and 3D4 (an IgG MAb) in the same assay.
Yadav, P., Carr, M. T., Yu, R., Mumbey‐Wafula, A., & Spatz, L. A. (2016). Mapping an epitope in EBNA‐1 that is recognized by monoclonal antibodies to EBNA‐1 that cross‐react with dsDNA. Immunity, inflammation and disease, 4(3), 362-375.
ELISA
Figure 8 Peptide mapping.
16D2 was tested by ELISA for binding to peptides spanning the entire 148 amino acid VBS region. Insets compare binding of 16D2 to EBNA-1 and PFM-15.
Yadav, P., Carr, M. T., Yu, R., Mumbey‐Wafula, A., & Spatz, L. A. (2016). Mapping an epitope in EBNA‐1 that is recognized by monoclonal antibodies to EBNA‐1 that cross‐react with dsDNA. Immunity, inflammation and disease, 4(3), 362-375.
ELISA
Figure 9 Peptide mapping.
(C) Comparison of amino acid sequence of homologous peptides GC-15, PFM-15, and GL-15. Common core region of all three peptides is underlined. Regions of overlap between GC-15 and PFM-15 and between PFM-15 and GL-15 are bolded. 3D4 (D) and 16D2 (E) were examined by ELISA for binding to GC-15, GL-15, and PFM-15. 3D4 was used at a concentration of 5 μg/ml and 16D2 at 10 μg/ml for all above ELISAs.
Yadav, P., Carr, M. T., Yu, R., Mumbey‐Wafula, A., & Spatz, L. A. (2016). Mapping an epitope in EBNA‐1 that is recognized by monoclonal antibodies to EBNA‐1 that cross‐react with dsDNA. Immunity, inflammation and disease, 4(3), 362-375.
ELISA
Figure 10 Peptide inhibition of MAbs binding to EBNA‐1.
16D2 (5 μg/ml) was incubated with increasing concentrations of peptides PFM-15 or GC-15 and examined by ELISA for binding to EBNA-1.
Yadav, P., Carr, M. T., Yu, R., Mumbey‐Wafula, A., & Spatz, L. A. (2016). Mapping an epitope in EBNA‐1 that is recognized by monoclonal antibodies to EBNA‐1 that cross‐react with dsDNA. Immunity, inflammation and disease, 4(3), 362-375.
ELISA
Figure 11 Peptide inhibition of MAbs binding to dsDNA.
16D2 (5 μg/ml) was incubated with increasing concentrations of peptides PFM-15 or GC-15 and examined by ELISA for binding dsDNA.
Yadav, P., Carr, M. T., Yu, R., Mumbey‐Wafula, A., & Spatz, L. A. (2016). Mapping an epitope in EBNA‐1 that is recognized by monoclonal antibodies to EBNA‐1 that cross‐react with dsDNA. Immunity, inflammation and disease, 4(3), 362-375.
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Specifications
- Immunogen
- rEBNA-1 in complete Freund's adjuvant
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgM, κ
- Specificity
- EBV EBNA1
- Species Reactivity
- EBV
- Clone
- 16D2
- Applications
- ELISA, WB, IF
- Related Disease
- the autoimmune disease, Systemic Lupus Erythematosus (SLE)
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Epstein-Barr virus EBNA-1; EBNA-1
- Gene ID
- 3783709
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF066 | DRB1*01:01/EBV EBNA1 (TSLYNLRRGTALA) MHC Monomer | MHC Multimer | |
| MHC-YF068 | DRB1*04:01/EBV EBNA1 (AEGLRALLARSHVER) MHC Monomer | MHC Multimer | |
| MHC-YF072 | DRB1*07:01/EBV EBNA1 (VYGGSKTSLYNLRRGTALAI) MHC Monomer | MHC Multimer | |
| MHC-YF177 | B*35:01/EBV EBNA1 (HPVGEADYFEY) MHC Monomer | MHC Multimer | |
| MHC-YF403 | B*35:01/EBV EBNA-1 (4A) (HPVAEADYFEY) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0523WJ | Mouse Anti-EBNA-1 Recombinant Antibody (clone 3D4) | ELISA, WB, IF | Mouse IgG, κ |
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