Human Anti-EBOV VP35 Recombinant Antibody (clone B10) (CAT#: VS-0323-CJ17)
This human anti-EBOV VP35 antibody (clone B10) is a product that recognizes EBOV VP35.
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ELISA
Figure 1 Reactivity of the anti-VP35 scFv clones in ELISA.
WB
Figure 2 Reactivity of the anti-VP35 scFv clones in immunoblot assay.
Recombinant VP35 protein and GO antigen as a control were analyzed by SDS-PAGE, blotted onto nitrocellulose membrane and cut into strips. Each strip was incubated with the supernatant deriving from a bacterial clone producing one of the anti-VP35 scFvs as indicated, and the antigen-antibody reaction revealed by ECL.
IA
Figure 3 ScFv against EBOV VP35 protein effect in the luciferase reporter gene assay.
Twenty-four hours after co-transfection with pGL IFN-β luc and pcDNA3 or pcDNA3 EBOV wtVP35 expression vector, cells were additionally transfected with different scFv p Target vectors and next day additionally transfected with IAV vRNA. The results from three independent experiments are shown as a percentage of the IFNβ promoter induction.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- EBOV VP35
- Species Reactivity
- EBOV
- Clone
- B10
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunoassay
- Conjugate
- Unconjugated
- Related Disease
- Ebola hemorrhagic fever
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The binding specificity of this scFv antibody to an active form of the Zaire EBOV VP35 was tested by Enzymed-linked Immunosorbent Assay (25-500 μg/mL), Western Blot and Immunoassay. Expressed as an cytosolic intrabody, it reversed the inhibition of type I IFN induction by intracellular expression of VP35.
Target
- Alternative Names
- VP35
- UniProt ID
- X5GXU1
- Sequence Similarities
- Belongs to the filoviridae polymerase cofactor VP35 family.
- Cellular Localization
- Host cytoplasm; Virion
- Function
- Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-500CQ | Recombinant Mouse Anti-EBOV VP35 Antibody (VC409) | WB, IP, IF | Mouse antibody |
| VS-0323-CJ16 | Human Anti-EBOV VP35 Recombinant Antibody (clone A10) | ELISA, WB, IA | Human IgG |
| VS-0323-CJ18 | Human Anti-EBOV VP35 Recombinant Antibody (clone E1) | ELISA, WB, IA, Inhib | Human IgG |
| VS-0323-CJ19 | Human Anti-EBOV VP35 Recombinant Antibody (clone F9) | ELISA, WB, IA, Inhib | Human IgG |
| VS-0323-CJ20 | Human Anti-EBOV VP35 Recombinant Antibody (clone H7) | ELISA, WB, IA | Human IgG |
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