Human Anti-EBOV VP35 Recombinant Antibody (clone B10) (CAT#: VS-0323-CJ17)
This human anti-EBOV VP35 antibody (clone B10) is a product that recognizes EBOV VP35.
Specific Inquiry
ELISA
Figure 1 Reactivity of the anti-VP35 scFv clones in ELISA.
WB
Figure 2 Reactivity of the anti-VP35 scFv clones in immunoblot assay.
Recombinant VP35 protein and GO antigen as a control were analyzed by SDS-PAGE, blotted onto nitrocellulose membrane and cut into strips. Each strip was incubated with the supernatant deriving from a bacterial clone producing one of the anti-VP35 scFvs as indicated, and the antigen-antibody reaction revealed by ECL.
IA
Figure 3 ScFv against EBOV VP35 protein effect in the luciferase reporter gene assay.
Twenty-four hours after co-transfection with pGL IFN-β luc and pcDNA3 or pcDNA3 EBOV wtVP35 expression vector, cells were additionally transfected with different scFv p Target vectors and next day additionally transfected with IAV vRNA. The results from three independent experiments are shown as a percentage of the IFNβ promoter induction.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- EBOV VP35
- Species Reactivity
- EBOV
- Clone
- B10
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunoassay
- Conjugate
- Unconjugated
- Related Disease
- Ebola hemorrhagic fever
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The binding specificity of this scFv antibody to an active form of the Zaire EBOV VP35 was tested by Enzymed-linked Immunosorbent Assay (25-500 μg/mL), Western Blot and Immunoassay. Expressed as an cytosolic intrabody, it reversed the inhibition of type I IFN induction by intracellular expression of VP35.
Target
- Alternative Names
- VP35
- UniProt ID
- X5GXU1
- Sequence Similarities
- Belongs to the filoviridae polymerase cofactor VP35 family.
- Cellular Localization
- Host cytoplasm; Virion
- Function
- Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "EBOV VP35"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1641CQ | Recombinant Mouse Anti-EBOV VP35 Antibody (10C7) | WB | Mouse antibody |
| VS-0323-CJ16 | Human Anti-EBOV VP35 Recombinant Antibody (clone A10) | ELISA, WB, IA | Human IgG |
| VS-0323-CJ18 | Human Anti-EBOV VP35 Recombinant Antibody (clone E1) | ELISA, WB, IA, Inhib | Human IgG |
| VS-0323-CJ19 | Human Anti-EBOV VP35 Recombinant Antibody (clone F9) | ELISA, WB, IA, Inhib | Human IgG |
| VS-0323-CJ20 | Human Anti-EBOV VP35 Recombinant Antibody (clone H7) | ELISA, WB, IA | Human IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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