Rabbit Anti-ELANE Recombinant Antibody (clone R01-6K1) (CAT#: VS3-XY3342)
It is a recombinant rabbit antibody that recognizes human ELANE (Elastase, neutrophil expressed), which inhibits C5a-dependent neutrophil enzyme release and chemotaxis. The antibody recognizes and binds to human ELANE. This antibody has been used in Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50-1/100, 1/50-1/100 and 1/50-1/200, respectively.
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Normal Tissue
RNA Expression
Figure 1 Bone marrow
(Hematopoietic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73774/170937_B_6_4.jpg
(Hematopoietic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73774/170937_B_6_4.jpg
Figure 2 RNA cell line category: Cell line enriched (THP-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197561-ELANE
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197561-ELANE
Figure 2 RNA cell line category: Cell line enriched (THP-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197561-ELANE
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197561-ELANE
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ELANE
- Species Reactivity
- Human
- Clone
- R01-6K1
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Cyclic Neutropenia
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
- Gene ID
- 1991
- UniProt ID
- P08246
- Long Name
- Elastase, neutrophil expressed
- Cellular Localization
- Cytoplasmic vesicle, phagosome
- Post Translation Modifications
- Glycosylation at Asn88, Asn124, and Asn173 (NX_P08246 [NX_P08246-1])
Modification sites at PhosphoSitePlus (P08246)
Glycosylation from GlyConnect ELNE_HUMAN (1564)
Glycosylation from GlyGen (P08246) 3 sites, 3 N-linked glycans (2 sites)
- Protein Refseq
- XP_054176121.1; XP_054176122.1
- Function
- Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes.
Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022).
Promotes cleavage of GSDMB, thereby inhibiting pyroptosis (PubMed:36899106).
Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1041 | Mouse Anti-ELANE Recombinant Antibody (clone YC039); Fab Fragment | ELISA | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
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