Rabbit Anti-ENDOG Recombinant Antibody (clone R04-1X6)
CAT#: VS3-XY3345
This rabbit recombinant antibody specifically recognizes ENDOG (Endonuclease G), which promotes conservative recombination when the DNA is 5hmC-modified. It reacts with human, mouse and rat ENDOG. It has been tested using Western Blot. The recommended dilutions of Western Blot is 1/500-1/1000.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21830/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21335/50074_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21335/50074_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21335/50074_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21335/50074_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21335/50074_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21335/50074_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167136-ENDOG
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ENDOG
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-1X6
- Applications
- WB
- Related Disease
- Colorectal Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
- Alternative Names
- Endonuclease G
- Gene ID
- 2021
- UniProt ID
- Q14249
- Long Name
- Endonuclease G
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- GSK3-beta-mediated dual phosphorylations at Thr-128 and Ser-288 is necessary for its interaction with YWHAG and the induction of autophagy. (Q14249-NUCG_HUMAN)
Ubiquitination at Lys66, Lys76, Lys153, Lys210, and Lys214 (NX_Q14249 [NX_Q14249-1])
Modification sites at PhosphoSitePlus (Q14249)
- Protein Refseq
- XP_011516649.2; XP_054218231.1
- Function
- Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA (PubMed:25355512).
The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs (By similarity).
Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs (By similarity).
Promotes conservative recombination when the DNA is 5hmC-modified (PubMed:25355512).
Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ENDOG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2787z | Mouse Anti-ENDOG Recombinant Antibody (clone 24F10) | WB, ELISA | Mouse IgG1 |
| MRO-0533-CN | Rabbit Anti-ENDOG Recombinant Antibody (clone JU31-30) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1149 | Hi-Affi™ Rabbit Anti-ENDOG Recombinant Antibody (clone DS1149AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2281 | Anti-ENDOG Immunohistochemistry Kit | IHC |
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