Rabbit Anti-FEN1 Recombinant Antibody (clone R02-8G3) (CAT#: VS3-XY3357)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat FEN1

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R02-8G3

• Applications
• WB, IHC-P

• Related Disease
• Werner Syndrome; Xeroderma Pigmentosum, Variant Type

• Clonality
• Monoclonal

• Aggregation
• < 5%

• Format
• Liquid

• Buffer
• PBS (pH 7.4), 150 mM NaCl and 50% glycerol

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• WB: 1/500-1/1000
  IHC: 1/50-1/100

• Alternative Names
• MF1; RAD2; FEN-1

• Gene ID
• 2237

• UniProt ID
• P39748

• Long Name
• Flap structure-specific endonuclease 1

• Cellular Localization
• Nucleus, nucleoplasm

• Post Translation Modifications
• Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA. (P39748-FEN1_HUMAN)
  Ubiquitination at Lys8, Lys171, Lys254, and Lys345 (NX_P39748 [NX_P39748-1])
  Modification sites at PhosphoSitePlus (P39748)
  Glycosylation from GlyGen (P39748) 2 sites, 1 O-linked glycan (2 sites)

• Protein Refseq
• NP_004102.1

• Function
• Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions.
  Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity.
  Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Secondary Antibody

• 2RD-A1473-YJ
• Goat Anti-Rabbit IgG-F(ab')2 Secondary antibody

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