Rabbit Anti-FEN1 Recombinant Antibody (clone R02-8G3) (CAT#: VS3-XY3357)
It is a recombinant rabbit antibody targeting the FEN1 (Flap structure-specific endonuclease 1). The antibody specifically reacts with human, mouse and rat FEN1. It has been reported for use in Western Blot and Immunohistochemistry-Paraffin. The recommended dilutions of western blot and Immunohistochemistry-Paraffin is 1/500-1/1000 and 1/50-1/100, respectively.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/710_G8_4_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/710_G8_4_selected.jpg
Figure 2 Tonsil
(High expression in tonsil)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6748/18132_A_4_8_val_selected.jpg
(High expression in tonsil)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6748/18132_A_4_8_val_selected.jpg
Figure 3 Testis
(Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/5862_A_5_6.jpg
(Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/5862_A_5_6.jpg
Figure 4 Vagina
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/5862_B_3_2.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/5862_B_3_2.jpg
Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/5862_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/5862_A_8_8.jpg
Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/5862_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/5862_A_9_4.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168496-FEN1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168496-FEN1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat FEN1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-8G3
- Applications
- WB, IHC-P
- Related Disease
- Werner Syndrome; Xeroderma Pigmentosum, Variant Type
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
Target
- Alternative Names
- MF1; RAD2; FEN-1
- Gene ID
- 2237
- UniProt ID
- P39748
- Long Name
- Flap structure-specific endonuclease 1
- Cellular Localization
- Nucleus, nucleoplasm
- Post Translation Modifications
- Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA. (P39748-FEN1_HUMAN)
Ubiquitination at Lys8, Lys171, Lys254, and Lys345 (NX_P39748 [NX_P39748-1])
Modification sites at PhosphoSitePlus (P39748)
Glycosylation from GlyGen (P39748) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_004102.1
- Function
- Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions.
Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity.
Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1282 | Hi-Affi™ Rabbit Anti-FEN1 Recombinant Antibody (clone DS1282AB) | WB, IHC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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