Rabbit Anti-FEN1 Recombinant Antibody (clone R02-8G3)
CAT#: VS3-XY3357
It is a recombinant rabbit antibody targeting the FEN1 (Flap structure-specific endonuclease 1). The antibody specifically reacts with human, mouse and rat FEN1. It has been reported for use in Western Blot and Immunohistochemistry-Paraffin. The recommended dilutions of western blot and Immunohistochemistry-Paraffin is 1/500-1/1000 and 1/50-1/100, respectively.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/710_G8_4_selected.jpg
Normal Tissue
Figure 2 Tonsil
High expression in tonsil
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6748/18132_A_4_8_val_selected.jpg
Normal Tissue
Figure 3 Testis
Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/5862_A_5_6.jpg
Normal Tissue
Figure 4 Vagina
Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/5862_B_3_2.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/5862_A_8_8.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2262/5862_A_9_4.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168496-FEN1
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat FEN1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-8G3
- Applications
- WB, IHC-P
- Related Disease
- Werner Syndrome; Xeroderma Pigmentosum, Variant Type
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
Target
- Alternative Names
- MF1; RAD2; FEN-1
- Gene ID
- 2237
- UniProt ID
- P39748
- Long Name
- Flap structure-specific endonuclease 1
- Cellular Localization
- Nucleus, nucleoplasm
- Post Translation Modifications
- Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA. (P39748-FEN1_HUMAN)
Ubiquitination at Lys8, Lys171, Lys254, and Lys345 (NX_P39748 [NX_P39748-1])
Modification sites at PhosphoSitePlus (P39748)
Glycosylation from GlyGen (P39748) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_004102.1
- Function
- Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions.
Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity.
Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-XY3357. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "FEN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3428z | Mouse Anti-FEN1 Recombinant Antibody (clone 19G12) | WB, ICC, IF, IP | Mouse IgG1 |
| FAMAB-1145CQ | Mouse Anti-FEN1 Recombinant Antibody (clone SAIC-21C-4) | Immuno-MRM, ELISA | Mouse IgG1 |
| ZG-0337J | Mouse Anti-FEN1 Recombinant Antibody (ZG-0337J) | WB, ICC | Mouse IgG |
| ZG-0338J | Mouse Anti-FEN1 Recombinant Antibody (ZG-0338J) | WB | Mouse IgG |
| VS3-QX420 | Mouse Anti-FEN1 Recombinant Antibody (clone 1E7-A6-H6) | WB, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1282 | Hi-Affi™ Rabbit Anti-FEN1 Recombinant Antibody (clone DS1282AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY828 | Anti-FEN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2499 | Anti-Mouse FEN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2498 | Anti-Human FEN1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: WB, ELISA, FuncS
Application: FuncS, Inhib, IP, ELISA
Application: ELISA, FC, WB, Block
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: IF, IP, Neut, FuncS, ELISA, FC
Application: ELISA, FC, Inhib, IHC-Fr, WB, IP
Application: WB, IHC-Fr, IHC-P, ICC, IF
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
