Recombinant Arabian camel Anti-FURIN Single Domain Antibody (PABC-557)
CAT#: PABC-557
This product is a recombinant Arabian camel antibody that can recognize FURIN. Nb14 binds at a site distant to the substrate binding pocket to the P-domain of furin. Nb14 inhibits furin cleavage of large substrates via steric exclusion.
Published Data
Gene Expression
IP
Figure 1 Co-immunoprecipitation of human furin with the different sdAbs RPE.
40 cells were transfected with human furin with or without expression vectors encoding the different sdAbs tagged with HA and immunoprecipitation was performed using an anti-HA antibody. Subsequently, Western blot analysis was performed using the anti-furin antibody MON152. Lanes 1, beads without anti-HA antibody; lanes 2, beads with anti-HA antibody; lanes 3, total cell lysate. Untransfected cells were included as a negative control in the last lane (-).
Dahms, S. O., Creemers, J. W., Schaub, Y., Bourenkov, G. P., Zögg, T., Brandstetter, H., & Than, M. E. (2016). The structure of a furin-antibody complex explains non-competitive inhibition by steric exclusion of substrate conformers. Scientific reports, 6, 34303.
Inhib
Figure 2 The sdAbs inhibit diphtheria-toxin-mediated cytotoxicity as efficiently as α1-PDX.
HEK-293T cells were transfected with empty vector, an expression vector encoding α1-PDX or the different sdAbs 24 h before exposure to diphtheria toxin. Then, 3 h after adding diphtheria toxin, cell viability was assessed by the MTT assay. The sdAbs protected the cells from cytotoxicity as efficiently as the well characterized furin inhibitor α1-PDX. The viability of cells treated with the diphtheria toxin together with α1-PDX or the different sdAbs is significantly higher when compared with cells only treated with the diphtheria toxin.
Dahms, S. O., Creemers, J. W., Schaub, Y., Bourenkov, G. P., Zögg, T., Brandstetter, H., & Than, M. E. (2016). The structure of a furin-antibody complex explains non-competitive inhibition by steric exclusion of substrate conformers. Scientific reports, 6, 34303.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9499/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Nucleoplasm & Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9499/992_E8_1_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67869/168556_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67869/168556_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67869/168556_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67869/168556_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000140564-FURIN
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Specifications
- Host Species
- Arabian camel
- Type
- Arabian camel VHH
- Specificity
- Human FURIN
- Species Reactivity
- Human
- Clone
- PABC-557
- Applications
- ELISA, Inhib, IP
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunoprecipitation and Inhibition. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- Furin, Paired Basic Amino Acid Cleaving Enzyme; Furin (Paired Basic Amino Acid Cleaving Enzyme); Paired Basic Amino Acid Residue-Cleaving Enzyme; EC 3.4.21.75; PCSK3; PACE; FUR; Paired Basic Amino Acid Cleaving Enzyme (Furin, Membrane Associated Receptor Protein); Proprotein Convertase Subtilisin/Kexin Type 3; Furin, Membrane Associated Receptor Protein; Dibasic Processing Enzyme; Dibasic-Processing Enzyme; FES Upstream Region; EC 3.4.21; SPC1
- Gene ID
- 5045
- UniProt ID
- P09958
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "FURIN"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-593-sdAb | Recombinant Anti-human FURIN VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2412MZ | Recombinant Mouse Anti-Human FURIN Antibody (clone NN0309-5H42) | IHC-P, WB | Mouse antibody |
| MRO-0613-CN | Rabbit Anti-FURIN Recombinant Antibody (clone JB35-53) | WB, IHC, IF | Rabbit IgG |
| ZG-0005U | Rabbit Anti-FURIN Recombinant Antibody (clone 1C3) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1364 | Hi-Affi™ Rabbit Anti-FURIN Recombinant Antibody (clone DS1364AB) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY873 | Anti-FURIN Immunohistochemistry Kit | IHC | |
| VS-0525-XY2669 | Anti-Human FURIN Immunohistochemistry Kit | IHC |
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