Recombinant Arabian camel Anti-FURIN Single Domain Antibody (PABC-557) (CAT#: PABC-557)

Figure 1 Co-immunoprecipitation of human furin with the different sdAbs RPE.

40 cells were transfected with human furin with or without expression vectors encoding the different sdAbs tagged with HA and immunoprecipitation was performed using an anti-HA antibody. Subsequently, Western blot analysis was performed using the anti-furin antibody MON152. Lanes 1, beads without anti-HA antibody; lanes 2, beads with anti-HA antibody; lanes 3, total cell lysate. Untransfected cells were included as a negative control in the last lane (-).

Dahms, S. O., Creemers, J. W., Schaub, Y., Bourenkov, G. P., Zögg, T., Brandstetter, H., & Than, M. E. (2016). The structure of a furin-antibody complex explains non-competitive inhibition by steric exclusion of substrate conformers. Scientific reports, 6, 34303.

Figure 2 The sdAbs inhibit diphtheria-toxin-mediated cytotoxicity as efficiently as α1-PDX.

HEK-293T cells were transfected with empty vector, an expression vector encoding α1-PDX or the different sdAbs 24 h before exposure to diphtheria toxin. Then, 3 h after adding diphtheria toxin, cell viability was assessed by the MTT assay. The sdAbs protected the cells from cytotoxicity as efficiently as the well characterized furin inhibitor α1-PDX. The viability of cells treated with the diphtheria toxin together with α1-PDX or the different sdAbs is significantly higher when compared with cells only treated with the diphtheria toxin.

Dahms, S. O., Creemers, J. W., Schaub, Y., Bourenkov, G. P., Zögg, T., Brandstetter, H., & Than, M. E. (2016). The structure of a furin-antibody complex explains non-competitive inhibition by steric exclusion of substrate conformers. Scientific reports, 6, 34303.