Mouse Anti-Ganglioside Recombinant Antibody (clone P3) (CAT#: HPAB-1183WJ)

This product is a mouse antibody targeting gangliosides containing N-glycolylated sialic acid.


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ELISA

Figure 1 Show the anti-anti-idiotypic (Ab1'') response against NeuGcGM3 measured by an indirect ELISA in the serum of mice immunized with P3 mAb coupled to KLH, and in the presence of Freund adjuvant.

Figure 1 Show the anti-anti-idiotypic (Ab1'') response against NeuGcGM3 measured by an indirect ELISA in the serum of mice immunized with P3 mAb coupled to KLH, and in the presence of Freund adjuvant.

ELISA

Figure 2 Shows the reactivity of the anti-idE1 Mab against E1, A3 and P3 anti-ganglioside mAbs.

Figure 2 Shows the reactivity of the anti-idE1 Mab against E1, A3 and P3 anti-ganglioside mAbs.

Activ

Figure 3 P3 mAb has the capacity to activate in vivo B-1a cells.

Figure 3 P3 mAb has the capacity to activate in vivo B-1a cells.

The ex vivo expression of CD25, CD69, and CD86 was evaluated by flow cytometry.

Martínez, D., Pupo, A., Cabrera, L., Raymond, J., Holodick, N. E., & Hernández, A. M. (2017). B-CD8+ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody. Journal of immunology research, 2017.

Activ

Figure 4 P3 mAb was able to recognize and activate in vitro B-2 cells.

Figure 4 P3 mAb was able to recognize and activate in vitro B-2 cells.

Splenic B-2 cells isolated from naïve BALB/c mice were incubated with biotinylated P3 or control IgM mAbs, and the binding was evaluated by flow cytometry using a PECy5-conjugated streptavidin.

Martínez, D., Pupo, A., Cabrera, L., Raymond, J., Holodick, N. E., & Hernández, A. M. (2017). B-CD8+ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody. Journal of immunology research, 2017.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Clone
  • P3
  • Applications
  • ELISA, Activ

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The Ganglioside antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Activation.

Target

  • Alternative Names
  • Ganglioside

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone P3"

See other products for "Ganglioside"

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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