Mouse Anti-GluA1 Recombinant Antibody (clone 11B8) (CAT#: PABS-0256)
This recombinant monoclonal antibody to Rat GluA1 is a Mouse monoclonal antibody that can be used for applications: ELISA.
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FSEC
Figure 1 Characterization of antibody fragments against AMPAR subunits by FSEC.
FSEC profiles of recombinant expressed GluA1-mKalama with 11B8 scFv (blue trace), 15F1 Fab (cyan trace), 5B2 Fab (green trace) and without antibody fragment (red trace), respectively, detecting mKalama/GFP fluorescence.
Zhao, Y., Chen, S., Swensen, A. C., Qian, W. J., & Gouaux, E. (2019). Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. Science, 364(6438), 355-362.
FSEC
Figure 2 Characterization of antibody fragments against AMPAR subunits by FSEC.
FSEC profiles of recombinant expressed GluA2-GFP with 11B8 scFv (blue trace), 15F1 Fab (cyan trace), 5B2 Fab (green trace) and without antibody fragment (red trace), respectively, detecting mKalama/GFP fluorescence.
Zhao, Y., Chen, S., Swensen, A. C., Qian, W. J., & Gouaux, E. (2019). Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. Science, 364(6438), 355-362.
FSEC
Figure 3 Characterization of antibody fragments against AMPAR subunits by FSEC.
FSEC profiles of recombinant expressed GluA3-GFP with 11B8 scFv (blue trace), 15F1 Fab (cyan trace), 5B2 Fab (green trace) and without antibody fragment (red trace), respectively, detecting mKalama/GFP fluorescence.
Zhao, Y., Chen, S., Swensen, A. C., Qian, W. J., & Gouaux, E. (2019). Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. Science, 364(6438), 355-362.
FSEC
Figure 4 Characterization of antibody fragments against AMPAR subunits by FSEC.
FSEC profiles of recombinant expressed GluA4-GFP (D) with 11B8 scFv (blue trace), 15F1 Fab (cyan trace), 5B2 Fab (green trace) and without antibody fragment (red trace), respectively, detecting mKalama/GFP fluorescence.
Zhao, Y., Chen, S., Swensen, A. C., Qian, W. J., & Gouaux, E. (2019). Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. Science, 364(6438), 355-362.
FSEC
Figure 5 Characterization of antibody fragments against AMPAR subunits by FSEC.
FSEC profiles of 11B8 scFv/GluA1-mKalama complexes. The black trace in each panel denotes uncomplexed receptor. The antibody fragment is mixed with corresponding AMPARs subunit in a 1:1 molar ratio. Apparent affinity of the complex was tested by diluting the 100 nM complex (blue) to 10 nM (magenta trace) and 1 nM (red) using buffer.
Zhao, Y., Chen, S., Swensen, A. C., Qian, W. J., & Gouaux, E. (2019). Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. Science, 364(6438), 355-362.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Rat GluA1
- Species Reactivity
- Rat
- Clone
- 11B8
- Applications
- ELISA
- Related Disease
- Psychotic disorders
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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