This recombinant monoclonal antibody to Rat GluA1 is a Mouse monoclonal antibody that can be used for applications: ELISA.
Host: Mouse
Isotype: Mouse IgG
Applications: ELISA
Figure 1 Characterization of antibody fragments against AMPAR subunits by FSEC.
FSEC profiles of recombinant expressed GluA1-mKalama with 11B8 scFv (blue trace), 15F1 Fab (cyan trace), 5B2 Fab (green trace) and without antibody fragment (red trace), respectively, detecting mKalama/GFP fluorescence.
Zhao, Y., Chen, S., Swensen, A. C., Qian, W. J., & Gouaux, E. (2019). Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. Science, 364(6438), 355-362.
Figure 2 Characterization of antibody fragments against AMPAR subunits by FSEC.
FSEC profiles of recombinant expressed GluA2-GFP with 11B8 scFv (blue trace), 15F1 Fab (cyan trace), 5B2 Fab (green trace) and without antibody fragment (red trace), respectively, detecting mKalama/GFP fluorescence.
Zhao, Y., Chen, S., Swensen, A. C., Qian, W. J., & Gouaux, E. (2019). Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. Science, 364(6438), 355-362.
Figure 3 Characterization of antibody fragments against AMPAR subunits by FSEC.
FSEC profiles of recombinant expressed GluA3-GFP with 11B8 scFv (blue trace), 15F1 Fab (cyan trace), 5B2 Fab (green trace) and without antibody fragment (red trace), respectively, detecting mKalama/GFP fluorescence.
Zhao, Y., Chen, S., Swensen, A. C., Qian, W. J., & Gouaux, E. (2019). Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. Science, 364(6438), 355-362.
Figure 4 Characterization of antibody fragments against AMPAR subunits by FSEC.
FSEC profiles of recombinant expressed GluA4-GFP (D) with 11B8 scFv (blue trace), 15F1 Fab (cyan trace), 5B2 Fab (green trace) and without antibody fragment (red trace), respectively, detecting mKalama/GFP fluorescence.
Zhao, Y., Chen, S., Swensen, A. C., Qian, W. J., & Gouaux, E. (2019). Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. Science, 364(6438), 355-362.
Figure 5 Characterization of antibody fragments against AMPAR subunits by FSEC.
FSEC profiles of 11B8 scFv/GluA1-mKalama complexes. The black trace in each panel denotes uncomplexed receptor. The antibody fragment is mixed with corresponding AMPARs subunit in a 1:1 molar ratio. Apparent affinity of the complex was tested by diluting the 100 nM complex (blue) to 10 nM (magenta trace) and 1 nM (red) using buffer.
Zhao, Y., Chen, S., Swensen, A. C., Qian, W. J., & Gouaux, E. (2019). Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. Science, 364(6438), 355-362.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
Submit a review or a question
There are currently no Customer reviews or questions for PABS-0256. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.