Mouse Anti-GOLGA5 Recombinant Antibody (VS3-FY2722)
CAT#: VS3-FY2722
This anti-GOLGA5 product is a mouse recombinant monoclonal antibody that has been carefully developed to target GOLGA5. The calculated molecular weight of the antigen is 83.0 kDa. It exhibits cross-reactivity with human, mouse and rat. This monoclonal antibody is potential for conducting research on Differentiated Thyroid Carcinoma and Congenital Disorder Of Glycosylation, Type Iig. The antibody can be utilized for detection of GOLGA5 by Western Blot.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/1834_F4_2_selected.jpg
Normal Tissue
Figure 2 Epididymis
High expression in epididymis
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_3_6_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000066455-GOLGA5
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Golgin A5 of human, mouse and rat origins
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 83.0 kDa.
- Related Disease
- Differentiated Thyroid Carcinoma and Congenital Disorder Of Glycosylation, Type Iig
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Target
- Alternative Names
- Golgin A5; Cell Proliferation-Inducing Gene 31 Protein; Golgi Autoantigen, Golgin Subfamily A, 5; Golgi Integral Membrane Protein 5; RET-Fused Gene 5 Protein; Golgin-84; RFG5
- Gene ID
- 9950
- UniProt ID
- Q8TBA6
- Cellular Localization
- Golgi apparatus membrane
- Post Translation Modifications
- Highly phosphorylated during mitosis.
Phosphorylation is barely detectable during interphase.
Modification sites at PhosphoSitePlus
Modification sites at neXtProt
- Protein Refseq
- XP_011535722.1; XP_047287977.1; XP_054233121.1; XP_054233122.1; NP_005104.4
- Function
- Stimulates the formation of Golgi stacks and ribbons.
Involved in intra-Golgi retrograde transport.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Secondary Antibody
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Isotype Control
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- Product Name
See other products for "GOLGA5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1748MZ | Recombinant Mouse Anti-Human GOLGA5 Antibody | WB | Mouse antibody |
| MRO-0683-CN | Mouse Anti-GOLGA5 Recombinant Antibody (clone 11-D7) | WB, IHC | Mouse IgG2a |
| VS3-WK1069 | Mouse Anti-GOLGA5 Recombinant Antibody (VS3-WK1069) | WB | Mouse IgG |
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