Mouse Anti-GOLGA5 Recombinant Antibody (VS3-FY2722) (CAT#: VS3-FY2722)
This anti-GOLGA5 product is a mouse recombinant monoclonal antibody that has been carefully developed to target GOLGA5. The calculated molecular weight of the antigen is 83.0 kDa. It exhibits cross-reactivity with human, mouse and rat. This monoclonal antibody is potential for conducting research on Differentiated Thyroid Carcinoma and Congenital Disorder Of Glycosylation, Type Iig. The antibody can be utilized for detection of GOLGA5 by Western Blot.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/1834_F4_2_selected.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/1834_F4_2_selected.jpg
Figure 2 Epididymis
(High expression in epididymis)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_3_6_val_selected.jpg
(High expression in epididymis)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_3_6_val_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_B_8_5.jpg
Figure 4 Colon
(Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_9_3.jpg
(Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_9_3.jpg
Figure 5 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_8_4.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_7_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_7_5.jpg
Figure 7 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_4_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_4_6.jpg
Figure 8 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_7_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/992/2274_A_7_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000066455-GOLGA5
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000066455-GOLGA5
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Golgin A5 of human, mouse and rat origins
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 83.0 kDa.
- Related Disease
- Differentiated Thyroid Carcinoma and Congenital Disorder Of Glycosylation, Type Iig
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Target
- Alternative Names
- Golgin A5; Cell Proliferation-Inducing Gene 31 Protein; Golgi Autoantigen, Golgin Subfamily A, 5; Golgi Integral Membrane Protein 5; RET-Fused Gene 5 Protein; Golgin-84; RFG5
- Gene ID
- 9950
- UniProt ID
- Q8TBA6
- Cellular Localization
- Golgi apparatus membrane
- Post Translation Modifications
- Highly phosphorylated during mitosis.
Phosphorylation is barely detectable during interphase.
Modification sites at PhosphoSitePlus
Modification sites at neXtProt
- Protein Refseq
- XP_011535722.1; XP_047287977.1; XP_054233121.1; XP_054233122.1; NP_005104.4
- Function
- Stimulates the formation of Golgi stacks and ribbons.
Involved in intra-Golgi retrograde transport.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "GOLGA5"
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1748MZ | Recombinant Mouse Anti-Human GOLGA5 Antibody | WB | Mouse antibody |
| MRO-0683-CN | Mouse Anti-GOLGA5 Recombinant Antibody (clone 11-D7) | WB, IHC | Mouse IgG2a |
| VS3-WK1069 | Mouse Anti-GOLGA5 Recombinant Antibody (VS3-WK1069) | WB | Mouse IgG |
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