Rabbit Anti-GOLM1 Recombinant Antibody (clone 9C2) (CAT#: ZG-0689J)

Figure 1 Rabbit Anti-GOLM1 Antibody (ZG-0689J) in WB

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate
All lanes: GOLM1 antibody at 1.3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46, 45 KDa
Observed band size: 80 KDa

Figure 2 Rabbit Anti-GOLM1 Antibody (ZG-0689J) in FC

Overlay histogram showing Hela cells stained with this product (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.