Mouse Anti-GPIIb/IIIa Recombinant Antibody (clone MA-16N7C2) (CAT#: HPAB-0044-YJ)
This product is a recombinant mouse antibody that recognizes human GPIIb/IIIa. Mab MA-16N7C2 can block fibrinogen. In addition, MA-16N7C2, potently inhibits platelet aggregation induced by ADP, thrombin, arachidonic acid, collagen, U46619, adrenaline and platelet-activating factor, whereas ristocetin-induced aggregation was unaffected.
Specific Inquiry
Inhib
Figure 1 In vitro Flow Studies in the Flow Chamber
Inhibition by 6B4-Fab and MA-16N7C2 of platelet-dependent surface coverage of collagen-coated coverslips as determined after 5-minute perfusion of whole blood at 1500 s−1. A and E, PBS control. B, 6B4-Fab (1.5 μg/mL). C, MA-16N7C2 (0.5 μg/mL). D, 6B4-Fab (1.5 μg/mL) plus MA-16N7C2 (0.5 μg/mL). F, 6B4-Fab (2.25 μg/mL). G, MA-16N7C2 (0.75 μg/mL). H, 6B4 Fab (2.25 μg/mL) plus MA-16N7C2 (0.75 μg/mL). Data are given as mean±SEM (n=4). *P<0.05 vs PBS control.
Wu, D., Meiring, M., Kotze, H. F., Deckmyn, H., & Cauwenberghs, N. (2002). Inhibition of platelet glycoprotein Ib, glycoprotein IIb/IIIa, or both by monoclonal antibodies prevents arterial thrombosis in baboons. Arteriosclerosis, thrombosis, and vascular biology, 22(2), 323-328.
Inhib
Figure 2 Effect of 6B4-Fab and MA-16N7C2 administration on template bleeding time in baboons.
Open square indicates 0.6 mg/kg 6B4; solid square, 2.0 mg/kg 6B4; solid circle, 0.1 mg/kg MA-16N7C2; open circle, 0.3 mg/kg MA-16N7C2; and open triangle, 0.6 mg/kg 6B4 mg/kg MA-16N7C2. Data represent mean±SEM. *P<0.05 vs pretreatment value.
Wu, D., Meiring, M., Kotze, H. F., Deckmyn, H., & Cauwenberghs, N. (2002). Inhibition of platelet glycoprotein Ib, glycoprotein IIb/IIIa, or both by monoclonal antibodies prevents arterial thrombosis in baboons. Arteriosclerosis, thrombosis, and vascular biology, 22(2), 323-328.
RIA
Figure 3 Rate of ¹²⁵I-MA-16N7C2 binding to resting (filled circles) and 25 μM ADP-stimulated gel-filtered platelets (open circles).
Deckmyn, H., Stanssens, P., Hoet, B., Declerck, P. J., Lauwereys, M., Gansemans, Y., ... & Vermylen, J. (1994). An echistatin‐like Arg‐Gly‐Asp (RGD)‐containing sequence in the heavy chain CDR3 of a murine monoclonal antibody that inhibits human platelet glycoprotein IIb/IIIa function. British journal of haematology, 87(3), 562-571.
Specifications
- Immunogen
- BALB/c mice were immunized by intraperitoneal injection of 0.5-3.0×108 gel-filtered platelets from a pool of eight healthy volunteers.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human GPIIb/IIIa
- Species Reactivity
- Human
- Clone
- MA-16N7C2
- Applications
- ELISA, WB, IP, Inhib, RIA
- Related Disease
- Platelet aggregation
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody MA-16N7C2 has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation, Inhibition, Radioimmunoassay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Target
- Alternative Names
- ITGA2B/ITGB3; CD41/CD61; MA-16N7C2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0044-YJ-S(P) | Mouse Anti-GPIIb/IIIa Recombinant Antibody (clone MA-16N7C2); scFv Fragment | ELISA, WB, IP | Mouse scFv |
| HPAB-2090-FY-S(P) | Human Anti-GPIIb/IIIa Recombinant Antibody (clone PDG8); scFv Fragment | Inhib | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0044-YJ-F(E) | Mouse Anti-GPIIb/IIIa Recombinant Antibody (clone MA-16N7C2); Fab Fragment | ELISA, WB, IP | Mouse Fab |
| HPAB-2090-FY-F(E) | Human Anti-GPIIb/IIIa Recombinant Antibody (clone PDG8); Fab Fragment | Inhib | Human Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1358CQ | Human Anti-GPIIb/IIIa Recombinant Antibody (clone PDG7) | DB, Inhib | Human IgG1 |
| HPAB-2090-FY | Human Anti-GPIIb/IIIa Recombinant Antibody (clone PDG8) | Inhib | Human IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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